Ligase

Q. Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

Q.

What is the phenotypic ratio of trihybrid cross?

Q.

What is the difference between DNA polymerase and RNA polymerase?

Q. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. What is the name of this step?

1. Staining
2. Elution
3. Separation
4. Differentiation

Q. The linking of antibiotic resistance gene with plasmid vector became possible with:

1. DNA polymerase
2. Exonuclease
3. DNA ligase
4. Endonuclease

Q. While isolating DNA from bacteria, which of the following enzymes is not required?

1. Lysozyme
2. Ribonuclease
3. Deoxyribonuclease
4. Protease

Q. Following is the sequence of amino acids coded by an mRNA. Predict the nucleotide sequence in the RNA: Met-Phe-Phe-Phe-Phe-Phe-Phe

Q.

Chimeric DNA is a

1. Gene clone

2. Recombinant DNA

3. DNA which contains uracil

4. DNA synthesized from RNA

Q. Hn-RNA is

1. 
   Heteronuclear RNA
2. 
   Useful RNA
3. 
   Heterogeneous nuclear RNA
4. 
   Homonuclear RNA

Q. Clover leaf model of tRNA was proposed by

1. Meselson
2. Fleming
3. Went
4. Holley

Q. Why is an antibody molecule represented as H2L2?

Q. Match column I with column II, and choose the correct combination from the options given below.

Column I	Column II
a. Hind II	1. Recognition sequence
b. pBR $322$	2. Restriction enzyme
c. GAATTC	3. Antibiotic resistance gene
d. $am{p}^R$	4. Cloning vector

1. a - 3, b - 1, c - 4, d - 2
2. a - 4, b - 3, c - 2, d - 1
3. a - 2, b - 4, c - 1, d - 3
4. a - 1, b - 2, c - 3, d - 4

Q. Mention any two chemical differences between DNA and RNA?

Q. Heterogeneous nuclear RNA is converted into mRNA by

1. Tailing
2. Splicing
3. Capping
4. All of the above

Q. PCR technique was invented by

1. Sanger
2. Boyer
3. Cohn
4. Karry Mullis

Q. In pBR $322$, recognition sequences, that are present on the same type of selectable marker

1. $BamHI,SalI$
2. $PstI,PvuI$
3. None of the above
4. Both (A) and (B)

Q.

RNA is the genetic material in:

1. Birds

2. Bacteria

3. Retrovirus

4. Protozoa

Q. Describe the roles of heat, primers and the bacterium Thermus aquaticus in the process of PCR. [3]

Q. In pBR322, how many common recognition sites are present?

1. 7
2. 8
3. 3
4. 4

Q. Ori is some specific sequence in the vector pBR 322. Ori also has the recognition sequence for the restriction enzyme

1. Pvu II
2. Hind III
3. Pst I
4. None of the above

Q. BamH I site in present at

1. $rop$
2. $am{p}^{R}gene$
3. $te{t}^{R}gene$
4. $Ori$

Q. In vector pBR 322, the ‘rop’ codes for the

1. Pvu II
2. proteins that involved in the replication of the restriction enzyme
3. Pvu I
4. proteins that involved in the replication of the plasmid

Q.

DNA staining by ethidium bromide is followed by the exposure to UV rays. Now DNA is seen as bands of

1. Blue colour
2. Orange colour
3. Both B and C
4. Red colour

Q. The final proof for DNA as the genetic material came from the experiments of

1. Hargobind Khorana
2. Avery, Mcleod and McCarty
3. Hershey and Chase
4. Griffith

Q. An antibiotic resistance gene in a vector usually help in the selection of

1. Transformed cells
2. Competent cells
3. Non-transformed cells
4. Non-recombinant cells

Q. snRNAs are transcribed with the help of

1. RNA polymerase III
2. RNA polymerase II
3. RNA polymerase I and II
4. RNA polymerase I

Q. Pollen grains can be stored for several years in liquid nitrogen having a temperature of

1. $-80C^{\circ }$
2. $-160C^{\circ }$
3. $-120C^{\circ }$
4. $-196C^{\circ }$

Q. The restriction enzymes are used in genetic engineering, because

1. They can cut DNA at specific base sequence
2. They can join different DNA fragments
3. They can degrade harmful proteins
4. They are nucleases that cut DNA at variable sites

Q. Match column I with column II, and choose the correct combination from the options given below.

	Column I		Column II
a.	Cloning vector	1.	Agrobacterium
b.	Ti-DNA	2.	pBR322
c.	Disarmed pathogen	3.	GAATTC
d.	Recognition sequence	4.	Transformation

1. $a-3,b-4,c-1,d-2$
2. $a-3,b-4,c-2,d-1$
3. $a-2,b-1,c-3,d-4$
4. $a-2,b-1,c-4,d-3$

Q. Protein helping in opening of DNA double helix in front of replication fork is
a. DNA gyrase
b. DNA polymerase 1
c. DNA Ligase
d. DNA topoisomerase
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