M13 Bacteriophage

Q. The bacteriophage used as vectors are ___ and Lambda Phage.

Q. Which of the following statements about the vector is incorrect?

1. Phages can be used as vectors
2. All vectors are plasmids
3. Vectors transport DNA into the host cell
4. A vector should always harbour a selectable marker

Q. How has the use of Agrobacterium as vectors helped in controlling Meloidogyne incognita infestation in tobacco plants? Explain in correct sequence. [3]

Q. Which of these are most widely used as a vector in genetic engineering?

1. Hormones
2. Plasmids
3. Enzymes
4. Ribosomes

Q.

Match list I with list II and select the correct option

1. a-2, b-4, c-1, d-5, e-3

2. a-4, b-3, c-5, d-2, e-1

3. a-2, b-4, c-5, a-1, e-3

4. a-3, b-4, c-5, d-1, e-2

Q. The bacteriophage used as vectors are ___ and Lambda Phage.

Q. Bacteriophages contain

1. ss DNA
2. ds DNA
3. ss RNA
4. ds RNA

Q. Read the given statements and select the correct option.
Statement 1: The tumour inducing plasmid (Ti plasmid) acts as a cloning vector in recombinant DNA technology.
Statements : The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.

1. Both statements 1 and 2 are correct
2. Statement 1 is correct but statement 2 is incorrect
3. Statement 1 is incorrect but statement 2 is correct
4. Both statements 1 and 2 are incorrect

Q. pBR322, which is frequently used as a vector for cloning gene in E. coli is a/an

1. Original bacterial plasmid
2. Modified bacterial plasmid
3. Viral genome
4. Transposon

Q. Which one of the following viruses has single stranded DNA and is used extensively as cloning vector in recombinant DNA technology?

1. TMV
2. Lambda phage
3. Adenovirus
4. $M_{13}$

Q. Silencing of mRNA has been used in producing transgenic plants resistant to _____________.

1. White rusts
2. Bacterial blights
3. Bollwroms
4. Nematodes

Q. The Ti plasmid, is often used for making transgenic plants. This plasmid is found in

1. Yeast as a 2 $\mu$m plasmid
2. Azotobacter
3. Rhizobium of the roots of leguminous plants
4. Agrobacterium

Q. Silencing of mRNA has been used in producing transgenic plants resistant to

1. Bollworms
2. Nematodes
3. White rusts
4. Bacterial blights

Q. The malarial parasite (Plasmodium) was first discovered in RBCs of a malarial patient by

1. Ronald Ross
2. Haldane
3. Antonie van Leeuwenhoek
4. Charles Laveran

Q. The bacteriophage used as vectors are ___ and Lambda Phage.

Q. Which of the following bacteria is used as a vector for plant genetic engineering?

1. Agrobacterium tumefaciens
2. Bacteriophages
3. Thermus aquaticus
4. Pyrococcus furiosus

Q. Explain the role of Ti plasmids in biotechnology.

Q. These are a group of very small intra-cellular bacteria. They are transmitted by arthropod vectors. Some of the diseases caused by them are typhus, trench fever and Q fever. Name these organisms.

1. Viroids
2. Bacteriods
3. Rickettsia
4. Mycoplasma

Q. Which of these are most widely used as a vector in genetic engineering?

1. Hormones
2. Plasmids
3. Enzymes
4. Ribosomes

Q. Vector for T-DNA is

1. Salmonella typhimurium
2. Thermits aquaticus
3. Agrobacterium tumefaciens
4. Escherichia coli
5. Bacillus thuringiensis

Q. Haemozoin formed in malaria is produced from

1. Haem of haemoglobin
2. Globin part of haemoglobin
3. Dead leucocytes
4. Cryptozoites

Q. With reference to the designing of vectors based on Ti and Ri plasmids, consider the following statements.
a. They are disarmed by removing the genes causing the disease
b. They carry sites for insertion of gene of interest.
c. They carry selectable markers.
Which of the statements given are correct?

1. $1$ and $2$ only
2. $2$ and $3$ only
3. $1$ and $3$ only
4. $1,2$ and $3$

Q. Which of the following is used as a best genetic vector in plants?

1. Bacillus thuringiensis
2. Agrobacterium tumefaciens
3. Pseudomonas putida
4. All of the the above

Q. Manipulating with nucleic acid is a trend in biotechnology.
(a) Name any one organism used as vector.
(b) What are DNA polymerase ?

Q. Which of the following methods of transferring DNA is useful in bacteria and plants, but not in animals?

1. Microinjection of DNA
2. Plasmid transfer
3. Bacteriophage infection
4. Laser irradiation of cells in a fluid containing DNA

Q. In pickles, infection is rare due to

1. Plasmolysis
2. Decrease in water potential by salt
3. Increase in osmotic potential by salt
4. Decrease in temperature by salt

Q. Each cycle of erythrocyte schizogony in Plasmodium malariae is completed in

1. 48 to 72 hours
2. 48 hours
3. 72 hours
4. None of the above

Q. While cloning vectors, which of the two will be performed by biotechnologists bacteriophages or plasmids. Justify with reason.