Molecular Probes

Q. Single stranded DNA or RNA, tagged with a radioactive molecule and is used to detect genes is called

1. probe
2. iRNA
3. primer
4. plasmid

Q. A singe strand of nucleic acid tagged with a radioactive molecule is called

1. vector
2. selectable marker
3. plasmid
4. probe

Q. The rate of migration of DNA in an agarose gel is affected by which of the following factor(s)?
I. Concentration of DNA in the sample loaded on gel
II. Conformation of DNA molecule
III. Size of the DNA fragment
IV. Source from where DNA has been isolated

1. Only I
2. II and III
3. I, II and IV
4. II, III and IV

Q. Southern Blotting uses a nitrocellulose membrane to absorb the DNA from the gel. What makes the DNA move towards the nitrocellulose membrane?

1. Movement of liquid in the gel tank towards the blotting paper
2. Nitrocellulose membrane is positively charged
3. Nitrocellulose membrane contains DNA probes to which DNA fragments attach
4. The gel is placed above the membrane, and gravity pulls the DNA below to the membrane

Q. A radioactively labelled Probe can be detected by

1. PCR
2. ELISA
3. Autoradiography
4. UV rays

Q. Single stranded DNA or RNA, tagged with a radioactive molecule and is used to detect genes is called

1. probe
2. iRNA
3. primer
4. plasmid

Q. DNA probes are used to detect the presence of nucleic acid sequences in DNA samples by hybridisation.

1. random
2. same
3. complementary

Q. DNA or RNA segment tagged with a radioactive molecule is called

1. vector
2. probe
3. clone
4. plasmid

Q. Which of the following statements is correct for molecular probes?

1. Used to detect the complementary sequence in nucleic acid samples
2. Used to introduce recombinant DNA into an animal cell
3. Used to cut DNA at random positions
4. Facilitate the multiplication of DNA

Q. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

1. Negatively charged fragments do not move
2. The larger the fragment size, the farther it moves
3. The smaller the fragment size, the farther it moves
4. Positively charged fragments move to farther end

Q.

What is an allele-specific PCR?

Q. A piece of nucleotide used to detect a gene of complementary sequence by hybridising with it, is called

1. DNA probe
2. protein probe
3. cDNA
   
4. All of the above
   

Q. A probe which is a molecule used to locate specific sequences in a mixture of DNA or RNA molecules could be __________________.

1. A single stranded RNA
2. A single stranded DNA
3. Either RNA or DNA
4. Can be ssDNA but not ssRNA

Q. DNA probes are used to detect the presence of nucleic acid sequences in DNA samples by hybridisation.

1. random
2. same
3. complementary

Q. A $\text{DNA}$ sample if first boiled for a few minutes and then cooled down then what will show :-

1. Initial rise and then fall in UV absorbance
2. Constant UV absorbance
3. Initial drop and then rise in UV absorbance
4. Steady drop in UV absorbance

Q. Which of the following is most commonly used genetic vector?

1. Phage
2. Plasmid
3. Mosquito
4. Bacterial genome

Q.

Give one means of requiring the ability to detect DNA.

Q. A single strand of nucleic acid tagged with a radioactive molecule is called a _____.

1. vector
2. selectable marker
3. plasmid
4. probe

Q. A singe strand of nucleic acid tagged with a radioactive molecule is called

1. vector
2. selectable marker
3. plasmid
4. probe

Q. It is a single stranded DNA or RNA, tagged with a radioactive molecule and is used to defect mutated genes.

1. RNAi
2. Probe
3. Plasmid
4. Primer

Q. What is DNA probe?

Q. DNA probes are used to detect the presence of nucleic acid sequences in DNA samples by hybridisation.

1. random
2. same
3. complementary

Q. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. What is the name of this step?

1. Staining
2. Elution
3. Separation
4. Differentiation

Q. If the plasma membrane of a cell gets damaged, how would the cell repair it? What is the process called?

Q. To identify the gene of interest in the gel matrix DNA __ are used.

Q. Which of the following technique can be used for gene transfer in the formation of transgenic plants?

1. Microinjection
2. Electroporation
3. Both A and B
4. Centrifugation

Q. Amount of DNA, RNA and protein simultaneously can be estimated by

1. Chromatography
2. Autoradiography
3. Ultrafilteration
4. Filtration

Q. A single strand of nucleic acid tagged with a radioactive molecule is called

1. vector
2. selectable marker
3. plasmid
4. probe

Q. DNA or RNA segment tagged with a radioactive molecular is called

1. Vector
2. Probe
3. Clone
4. Plasmid