Obtaining Desired Gene

Q. Read the following statement having two blanks A and B.
In ”____A____, ____B____” an American Company prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of Escherichia coli to produce insulin chains.”
Identify the correct option for the two blanks.

1. A - 1983 , B - Eli Lilly
2. A - 1982, B - Eli Lily
3. A - 1985, B - Eli Lilly
4. A - 1986, B - Eli Lily

Q.

Is DNA positively charged?

Q. Production of human growth hormone in bacteria is possible because

1. bacteria has genes similar to humans
2. gene regulation is identical in humans and bacteria
3. bacteria have rapid growth rate
4. the genetic code is nearly universal

Q. Transcribed regions present in mature mRNA are ____(i)______ and transcribed regions not present in mature mRNA are _____(ii)______.

1. (i)-introns, (ii)-exons
2. (i)-exons, (ii)-introns
3. (i)-codons, (ii)-anticodons
4. (i)-anticodons, (ii)-codons

Q. Transcribed regions present in mature mRNA arise from ---(i)-- and transcribed regions not present in mature mRNA arise from ----ii----.

1. i. intron, ii. exon
2. i. exon, ii. intron
3. i. codon, ii. anticodon
4. i. anticodon, ii. codon

Q. Choose the correct answer from the alternatives given :
Select the correct statements regarding the process of transcription in eukaryotes.
(i) The strand of DNA which takes part in transcription process is called as coding strand.
(ii) The enzyme .RNA polymerase can catalyse polymerisation only in one direction i.e.., 5 $\to$ 3.
(iii) An unusual nucleotide methyl guanosine triphosphate is added to the 5 end of hnRNA during capping.
(iv) During Tailing Process. adenylate residues (200 - 300) are Added at 3 end in a template at independent manner.

1. (i) and (ii)
2. (ii); (iii) arid (iv)
3. (iii) and (iv)
4. (i), (ii), (iii) and (iv)

Q. Given below are some statements about satellite DNA.
(A) These are interspersed sequences.
(B) They are tandemly arranged.
(C) These are non-coding.
(D) These are unique sequences of DNA.

Choose the option which mentions the correct statements.

1. B and C
2. A and D
3. A and C
4. B and D

Q. Explain the process of making heterogenous nuclear RNA (hnRNA) into a fully functional mRNA in eukaryotes. Where does this process occur in the cell? [3 Marks]

Q. Which of the following contributes to change in DNA's buoyant density?

1. Operon
2. Intergenic sequence
3. Base composition
4. Cell size

Q.

Are exons junk DNA?

Q. The non protein coding regions in DNA are known as whereas the protein coding regions are known as .

1. exons
2. introns
3. genes
4. cDNA

Q. What is the function of the codon AUG?

Q. Transcribed regions present in mature mRNA arise from ---(i)-- and transcribed regions not present in mature mRNA arise from ----ii----.

1. i.codon , ii. anticodon
2. i.anticodon, ii. codon
3. i. intron , ii. exon
4. i. exon , ii. intron

Q. Assertion :Hn mRNA is larger than mRNA. Reason: HnRNA has nontranslating introns and more exons than required for translation.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. Nucleotides/nucleic acids occur in

1. Mitochondria
2. DNA, RNA, chloroplasts and nucleus
3. Ribosomes
4. All the above

Q. Which of the following is not true of RNA processing?

1. Exons are excised and hydrolysed before mRNA moves out of the nucleus.
2. An initial RNA transcript is much longer than the final RNA molecule that may leave the nucleus.
3. RNA splicing may be catalyzed by spliceosomes.
4. RNA splicing only takes place in eukaryotic nucleus.

Q. Statement A: The primary transcript produced in eukaryotes is translated without undergoing any modification or processing.
Statement B: The hnRNA in humans has exons and introns.

1. Both the statements A and B are correct.
2. Both the statements A and B are wrong.
3. Statement B is correct and statement A is wrong.
4. Statement A is correct and statement B is wrong.

Q. Introns are found in the circular chromosomes of prokaryotes.

1. True
2. False

Q. In which of the following organisms supercoiled DNA can be traced?

1. Prokaryotes
   
2. Eukaryotes
3. Bacteria
4. All of the above

Q. Transcribed regions present in mature mRNA arise from ---(i)-- and transcribed regions not present in mature mRNA arise from ----ii----.

1. i. intron , ii. exon
2. i. exon , ii. intron
3. i.codon , ii. anticodon
4. i.anticodon, ii. codon

Q. The non protein coding regions in DNA are known as whereas the protein coding regions are known as .

1. exons
2. introns
3. genes
4. cDNA

Q. what is untranslatd region on mRNA?

Q. Does mRNA have codon or anti-codons?

Q. The non protein coding regions in DNA are known as whereas the protein coding regions are known as .

1. exons
2. introns
3. genes
4. cDNA

Q. Some of the steps involved in the production of human insulin are given below. Choose the correct sequence
i. Obtaining of gene for human insulin using c-DNA technology
ii. Culturing recombinant E-coli in bioreactors
iii. Purification of human insulin
iv. Insertion of human insulin gene into plasmid
v. Introduction of recombinant plasmid into E.coli
vi. Extraction of recombinant gene product from E-coli

1. ii-i-iv-iii-v-vi
2. i-iii-v-vi-ii-iv
3. i-iv-v-ii-vi-iii
4. iii-v-ii-i-vi-iv