Obtaining Gene Product

Q. Which of the following organisms is used in the production of human insulin by genetic engineering?

1. Escherichia coli
2. Agrobacterium tumefaciens
3. Bacillus thuringiensis
4. Saccharomyces cerevisiae

Q. Select the incorrect statement with reference to transgenic animals.

1. The animals that have had their DNA manipulated are termed as transgenic animals
2. Human protein, α-1-antitrypsin is used to treat emphysema
3. Transgenic mice are being used to test the safety of the polio vaccine
4. The first transgenic cow, Rosie, produced human lactase enriched milk (2.4 g/litre)

Q. Oncogenes are

1. Extra genes sometimes present in certain eukaryotes
2. Protogenes which due to mutation have lost their ability to maintain a normal rate of cell division
3. Extra genes that get incorporated into the embryo during fertilisation
4. Additional genes present in an individual do not have an impact on cell division

Q. Identify a character that is not desirable in a cloning vector ?

1. An origin of replication site
2. An active promoter
3. One or more unique restriction endonuclease sites
4. Selectable markers such as genes for antibiotic resistance

Q. Regarding assertion and reason, select the correct option:

Assertion: Extraction and purification of enzyme is laborious and expensive
Reason: Protein engineering can be used to produce enzymes at large scale.

1. Both the assertion and reason are true and the reason is the correct explanation for the assertion.
2. Both the assertion and reason are true but the reason is not the correct explanation of the assertion.
3. Assertion is true but the reason is false
4. Both the assertion and reason are false.

Q. Which of the following is the correct sequence to artificially synthesise human insulin in E.coli?

A. Creation of disulfide bonds between A and B chains
B. Ligation of A and B genes with plasmid vector
C. Extraction of product from the bacteria
D. Transformation of E.coli with the recombinant vector
E. Culture of bacterial cells having recombinant DNA in the bioreactors
F. Synthesis of DNA sequences coding for A and B insulin peptide chains

1. B→F→D→A→C→E
2. F→B→D→A→E→C
3. A→B→D→E→C→F
4. F→B→D→E→C→A

Q. What do we mean by heterologous host?

Q. Which of the following is not a cloning vector?

1. Cosmid
2. Which has only one restriction site
3. pBR322
4. Whose origin supports high copy number

Q. Select the statement which is correct about viroids.

1. They range in size from $266-450$ nucleotides
2. They show a lot of sequence homology
3. They were discovered by Karshan in $1966$
4. The genome of viroids is larger than viruses

Q. Assertion :Biotechnology produces transgenic microorganisms that are micro-factories for proteins. Reason: Transgenic microorganisms can be developed to produce proteins of human use. E.g. - insulin.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. Choose the correct answer from the alternatives given :
The process of transformation is not affected by which of the following enzymes?
A. DNase, B. RNase, C. Peptidase, D. Lipase

1. A, B
2. A, B, C, D
3. B, C, D
4. A, B, C

Q. What could the mass culture of transgenic organisms achieve?

1. More organism for research purpose..
2. More organism that can survive the harsh environment
3. Industrial scale production of protein that is encoded by the transgene
4. All the above.

Q. What could the mass culture of transgenic organism achieve?

1. More organism for research purpose..
2. More organism that can survive the harsh environment
3. Industrial scale production of protein that is encoded by the transgene
4. All the above.

Q. What is the main advantage of producing genetically engineered insulin?

Q. Genetically-engineered human insulin is obtained by inserting the gene in __________.

1. Pancreatic cells
2. Agrobacterium tumafaciens
3. E.coli
4. Drosophila melanogaster

Q.

Transgenic organisms are produced by

1. deleting sex chromosomes
2. inducing gene mutations
3. introducing foreign genes
4. arresting spindle fibre formation

Q. Basically how many types of enzymes have been recognised by International Union of Biochemistry?

1. 4
2. 5
3. 6
4. 8

Q. Type of gene mutation which involves replacement of purine with pyrimidine or vice versa or the substitution of one type of base with another type of base is

1. Transduction
2. Translocation
3. Transcription
4. Transversion

Q. Genetically engineered human insulin is called as

1. Humulin
2. Haematin
3. Hybridoma
4. Hybrid

Q. What could the mass culture of transgenic organisms achieve?

1. More organism for research purpose..
2. More organism that can survive the harsh environment
3. Industrial scale production of protein that is encoded by the transgene
4. All the above.

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Select the correct sequence of cloning method.

1. V - IV - III - II - I
2. I - II - III - IV - V
3. III - V - II - IV - I
4. IV - II - I - V - III
5. II - IV - I - III - V

Q. What could the mass culture of transgenic organisms achieve?

1. More organism for research purpose..
2. More organism that can survive the harsh environment
3. Industrial scale production of protein that is encoded by the transgene
4. All the above.

Q. How did Avery, McLeod and McCarty prove that DNA was the genetic material. Explain.

Q. The transforming substance in Griffith's experiment is

1. protein
2. DNA
3. mRNA
4. polysaccharide

Q. Regarding assertion and reason, select the correct option:

Assertion: Extraction and purification of enzyme is laborious and expensive
Reason: Protein engineering can be used to produce enzymes at large scale.

1. Both the assertion and reason are true and the reason is the correct explanation for the assertion.
2. Both the assertion and reason are true but the assertion is not the correct explanation of the reason.
3. Assertion is true but the reason is false
4. Both the assertion and reason are false.

Q. What are the objectives of transferring the rDNA into a living host?

1. To increase the copy of rDNA naturally
2. To start the formation of protein that the gene of interest encodes
3. To make the rDNA to move along the generation of the host organism
4. All the above