Open and Closed Fermenter

Q. If u have a dense laboratory culture of bacteria in a petri dish what is the best measure to report its density?

Q. Stirrer in stirred tank type bioreactor facilitates

1. Temperature control
2. Foam control
3. pH control
4. Mixing and aeration

Q.

Stirred-tank bioreactors have been designed for

1. Availability of oxygen throughout the process

2. Ensuring anaerobic conditions in the culture vessel

3. Purification of the product

4. Addition of preservatives to the product

Q.

Why is agitation required in bioreactor?

Q. Which one of the following statements is true for cockroach?

1. The larval stage is called caterpillar
2. Anal styles are absent in females
3. They are ureotelic
4. The number of ovarioles in each ovary are ten.

Q. Atlas 66 has

1. high carbohydrate content
2. high protein content
3. high fatty acid content
4. low fatty acid content

Q. Stirred-tank bioreactors are defined for:

1. Availability of O${}_2$ throughout the process
2. Addition of preservative
3. Purification of the product
4. None of these

Q. What is the significance of creation of bubbles of bioreactor and how the bubbles are created

Q.

A bioreactor is:

1. Culture containing radioactive isotopes

2. Hybridoma

3. Fermentation tank

4. Culture for synthesis of new chemicals

Q. Upstream processes include

1. Separation
2. Biosynthesis
3. Purification
4. Preservation

Q. What is the major difference between simple stirred tank bioreactor and sparged stirred tank bioreactor? What is its advantage?

Q. Double fertilisation and triple fusion were discovered by

1. Hofmeister
2. Leeuwenhoek
3. Nawaschin and Guignard
4. Strassburger

Q. _______ is a device in which large volumes of living cells are cultured in order to obtain a specific product.

1. PCR
2. Incubator
3. Agitator
4. Bioreactor

Q. Stirred-tank bioreactors have advantages over shake flasks because they _______________.

1. Do not allow the entry of $C{O}_2$
2. Provide high temperature and pH
3. Provide better aeration and mixing properties
4. Are easy to operate

Q. Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.

Q. What are the advantages of stirred tank bioreactors over Shake flasks?

Q. One of the most common problem using yeast as a cell for producing a protein product is the contamination of bacteria. If a protein product has to be produced through yeast, what could be the solution to ensure that bacterial contamination is avoided.

1. Using closed type fermenter for yeast culturing
2. Usage of bacteria to produce the protein instead of yeast
3. Both B and C
4. Sterlizing the lab before innoculation of the yeast into the media.

Q. The process of separation and purification of expressed protein before marketing is called

1. downsteam processing
2. upstream processing
3. bioprocessing
4. post-production processing

Q. Difference between simple stirred tank bioreactor and sparged stirred tank bioreactor

Q. Sketch and label tubular tower fermenter.

Q. Identify the correct match for the given apparatus.

1. Apparatus- Gene gun, Function- Vectorless direct gene transfer
2. Apparatus- Column chromatograph, Function- Separation of chlorophyll pigments
3. Apparatus- Stirred tank bioreactor, Function- Carry out fermentation process
4. Apparatus- Respirometer, Function- Finding out rate of repiration

Q. Stirred-tank bioreactors have been designed for

1. Availability of oxygen throughout the process
2. Ensuring anaerobic conditions in the cultural vessel
3. Purification of product
4. Addition of preservatives to the product

Q. Stirred-tank bioreactors have been designed for ?

1. Availability of Oxygen throughout the process
2. Addition of preservatives to the product
3. Purification of the product
4. Ensuring anaerobic conditions in the culture vessel

Q. Batch Fermentation:

1. Utilizes continuous draining of used medium from one side and adding fresh medium from other to maintain the cells in their physiologically most active log exponential phase
2. Maintain the cells in log phase
3. Is a type of closed system
4. Is same as continuous culture system

Q. Stirred tank bioreactors have been designed for:

1. Availability of oxygen through the process
2. Ensuring anearobic condition in the culture vessel
3. Addition of preservation to the product
4. Purification of product