Preparation of Media

Q. In the preparation of culture media for tissue culture, __________ is used as a solidifying agent.

1. carbohydrates
2. agar agar
3. amino acids
4. growth hormones

Q. Sterilization of the nutrient medium in tissue culture is done by ______________ method.

1. autoclaving
2. adding agar agar
3. fumigation
4. chemical sterilization
   

Q.

What is a bacterial smear?

Q. Match the following columns and select the correct option.

(a)	Monascus purpureus	(i)	Streptokinase
(b)	Trichoderma polysporum	(ii)	Autotrophic N${}_2$ fixer
(c)	Streptococcus	(iii)	Statins
(d)	Nostoc	(iv)	Cyclosporin A

1. (a) (b) (c) (d)
   (iv) (iii) (ii) (i)
2. (a) (b) (c) (d)
   (ii) (iii) (i) (iv)
3. (a) (b) (c) (d)
   (iii) (iv) (i) (ii)
4. (a) (b) (c) (d)
   (iv) (iii) (i) (ii)

Q.

Shashi and her friends wonder if pond water smells bad because it has microorganisms in it. Describe a way they could find out this.

Q. In a microbiology lab, a technician uses heat to sterilize the nutrient solution that is used to grow a fungus. When the heating system breaks down he sterilizes with the help of a filter, with a pore size of 0.2 micrometers. When the fungus was grown it didn't grow properly and looked unhealthy. What is the most likely reason?

1. The nutrient solution had a virus
2. Heating makes the glucose in the nutrient solution more digestible
3. Filtering removed some nutrients
4. The nutrient solution contained a bacterium that was pathogenic to fungus

Q.

Which among the following is the most sensitive and accurate test for the detection of very minute amount of hormones

1. Flurometricassay

2. Bioassay

3. Ascorbic acid depletion test

4. Immunoassay

Q. Identify the set of substances which are not used in the preparation of nutrient culture medium.

1. Abscisic acid, antibodies, enzymes
2. Sucrose, inorganic salts, vitamins
3. Sugars, mineral salts, growth regulators
4. Amino acids, auxins, cytokinins

Q. Match the following:

A	B
1. Sucrose	a. Micronutrient
2. Thiamine	b. Carbon source
3. Manganese	c. Macronutrient
4. Calcium	d. Vitamin

1. 1 - b, 2 - a, 3 - d, 4 - c
2. 1 - b, 2 - d, 3 - a, 4 - c
3. 1 - c, 2 - d, 3 - c, 4 - a
4. 1 - d, 2 - c, 3 - a, 4 - b

Q. In a microbiology lab, a technician uses heat to sterilize the nutrient solution that is used to grow a fungus. When the heating system breaks down he sterilizes with the help of a filter, with a pore size of 0.2 micrometers. When the fungus was grown it didn't grow properly and looked unhealthy. What is the most likely reason?

1. The nutrient solution had a virus
2. Heating makes the glucose in the nutrient solution more digestible
3. Filtering removed some nutrients
4. The nutrient solution contained a bacterium that was pathogenic to fungus

Q. In a microbiology laboratory, the technician uses heat to sterilise the nutrient solution that is used to grow 4. a fungus. When the heating system broke down, he sterilised the solution by passing It on a sterile environment) through a sterile filter with a pore size of 0.2 micrometers. When the fungus was grown on the filtered nutrient solution, it stopped growing and looked unhealthy within a few days. Which statements is the most likely explanation for the observed effects on the fungus ?

1. The nutrient solution contained a virus
2. Heating makes the glucose in the nutrient solution more digestible
3. Filtering removed one of the larger nutrients
4. The nutrient solution contained a bacterium that was pathogenic to the fungus.

Q.

In a microbiology laboratory, the technician used heat to sterilize the nutrient solution that is used to grow a fungus. When the heating system broke down, he sterilized the solution by passing it (in a sterile environment) through a sterile filter with a pore size of 0.2 micrometers. When the fungus was grown on the filtered nutrient solution it stopped growing and looked unhealthy within a few days. Which statement is the most likely explanation for the observed effects on the fungus?

1. The nutrient solution contain a virus

2. Heating makes the glucose in the nutrient solution more digestible

3. Filtering removed one of the larger nutrient molecules

4. Filtering changed the pH of the nutrient solution