Process of DNA Fingerprinting

Q.

If there are 999 bases in an RNA that codes for a protein with 333 amino acids, and the bases at position 901 are detected such that the length of the RNA becomes 998 bases, how many codons will be altered?

Q.

What is satellite DNA? Explain.

Q.

What is the role of transacetylase in lac operon?

Q. The non-staining secondary constrictions at a constant location in few chromosomes that gives the appearance of a small fragment is called

1. telomere
2. centromere
3. kinetochores
4. satellite

Q. Match column-I with column-II and choose the correct option:

$\begin{array}{cccc}& \text{Column-I}& & \text{Column-II}\\ \text{(a)}& \text{VNTR}& \text{(i)}& \beta -\text{galactosidase}\\ \text{(b)}& \text{Lac operon}& \text{(ii)}& \text{DNA ligase}\\ \text{(c)}& \text{Genetic code}& \text{(iii)}& \text{DNA fingerprinting}\\ \text{(d)}& \text{Okazaki fragments}& \text{(iv)}& \text{Unambiguous}\end{array}$

1. a $\to$ iv, b $\to$ ii, c $\to$ iii, d $\to$ i
2. a $\to$ iii, b $\to$ i, c $\to$ iv, d $\to$ ii
3. a $\to$ iii, b $\to$ iv, c $\to$ i, d $\to$ ii
4. a $\to$ i, b $\to$ ii, c $\to$ iii, d $\to$ iv

Q.

What are the pros and cons of DNA fingerprinting?

Q. Conditions of a karyotype 2n + 1 and 2n + 2 is called

1. Aneuploidy
2. Polyploidy
3. Allopolyploidy
4. Monosomy

Q. Enzyme required for joining DNA fragments is

1. DNA polymerase
2. Adenosine deaminase
3. Topoisomerase
4. DNA ligase

Q. Which of the following is not an application of DNA fingerprinting?

1. Settling cases of disputed parentage
2. Solving criminal cases
3. PCR
4. Verifying the identity of an individual

Q.

Is there DNA in dead skin?

Q. Match column-I with column-II and choose the correct option:

$\begin{array}{cccc}& \text{Column-I}& & \text{Column-II}\\ \text{(a)}& \text{VNTR}& \text{(i)}& \beta -\text{galactosidase}\\ \text{(b)}& \text{Lac operon}& \text{(ii)}& \text{DNA ligase}\\ \text{(c)}& \text{Genetic code}& \text{(iii)}& \text{DNA fingerprinting}\\ \text{(d)}& \text{Okazaki fragments}& \text{(iv)}& \text{Unambiguous}\end{array}$

1. a $\to$ iv, b $\to$ ii, c $\to$ iii, d $\to$ i
2. a $\to$ iii, b $\to$ i, c $\to$ iv, d $\to$ ii
3. a $\to$ i, b $\to$ ii, c $\to$ iii, d $\to$ iv
4. a $\to$ iii, b $\to$ iv, c $\to$ i, d $\to$ ii

Q.

Which of the following can be used as samples to extract DNA for DNA fingerprinting?

1. Hair strands
2. Fingerprints
3. Blood samples
4. Footprints

Q. In the Trp operon of bacteria, if the concentration of Tryptophan is high, then

1. Attenuator shows green signal for the transcription of tryptophan structural genes
2. Attenuator facilitates elongation step of transcription
3. Attenuator restricts transcription
4. All of the above

Q. __ blotting method is used in DNA fingerprinting.

Q. Arrange the following steps of formation of recombinant DNA in the correct sequence.
I. Complementary sticky ends are produced on the foreign DNA and the plasmid.
II. Foreign DNA and plasmids are mixed together
III. DNA ligase is used to seal the nicks in the sugar–phosphate backbone.
IV. Cut the foreign DNA (containing the DNA fragment of interest) and the plasmid with the same restriction enzyme.

1. II, IV, III, I
2. I, IV, II, III
3. IV, II, I, III
4. IV, I, II, III

Q. Explain the significance of satellite DNA in DNA fingerprinting technique. [3]

Q. Gene cloning is :

1. Gene is isolated and inserted in same organism
2. Gene is isolated and inserted in different organism
3. Gene is isolated and inserted in plasmid of other organism
4. Gene is isolated and inserted in chromosomal DNA

Q.

What is meant by copy number?

Q. What is polymorphism and single nucleotide polymorphism ??

Q. Match column-I with column-II and choose the correct option:

$\begin{array}{cccc}& \text{Column-I}& & \text{Column-II}\\ \text{(a)}& \text{VNTR}& \text{(i)}& \beta -\text{galactosidase}\\ \text{(b)}& \text{Lac operon}& \text{(ii)}& \text{DNA ligase}\\ \text{(c)}& \text{Genetic code}& \text{(iii)}& \text{DNA fingerprinting}\\ \text{(d)}& \text{Okazaki fragments}& \text{(iv)}& \text{Unambiguous}\end{array}$

1. a $\to$ iv, b $\to$ ii, c $\to$ iii, d $\to$ i
2. a $\to$ iii, b $\to$ i, c $\to$ iv, d $\to$ ii
3. a $\to$ i, b $\to$ ii, c $\to$ iii, d $\to$ iv
4. a $\to$ iii, b $\to$ iv, c $\to$ i, d $\to$ ii

Q.

What Is Ligation-mediated PCR And Inter Sequence-specific PCR?

Q.

Why does DNA fingerprinting form the basis of paternity tests?

Q.

Is there DNA in fingernails?

Q. Select the incorrect statement about dNTPs.

Q. (a)The steps in DNA finger printing are given. Complete the flow chart (A & B).
(b) Mention the applications of DNA fingerprinting.

Q. The technique of DNA fingerprinting was invented by

1. Karl Mullis
2. Alec Jeffreys
3. Tatum and Beadle
4. Purkinje

Q. Exlpain DNA polymorphism as the basis of genetic mapping of human genome.

Q. Based on the position of the centromere, identify the type of chromosome shown in the figure.

1. Telocentric
2. Acrocentric
3. Metacentric
4. Submetacentric

Q. Which one of the following cannot be used for DNA fingerprinting in humans?

1. Sperms
2. Erythrocytes/RBCs
3. Hair bulbs
4. Leucocytes

Q.

What are genomic signatures?