Restriction Enzyme

Q. In one polynucleotide strand of a DNA molecule, the ratio of A+T/G+C is 0.3. What is the A+G/T+C ratio of the entire DNA molecule?

1. 1
2. 0.3
3. 0.7
4. 1.2

Q. Spliceosomes are not found in cells of

1. Fungi
2. Bacteria
3. Animals
4. Plants

Q. A restriction endonuclease breaks bonds between the

1. sugar and phosphate components of a nucleic acid
2. exons and introns of a DNA
3. base pairs of a DNA
4. base pairs of a DNA-RNA hybrid

Q. The number of phosphodiester bonds and hydrogen bonds broken due to action of EcoRI at one recognition sequence?

1. 
2. 
3. 
4. 

Q.

The net electric charge on DNA and histones is

1. Zero

2. Negative and positive, respectively

3. Both positive

4. Both negative

Q. Which of the following statements does not hold true for restriction enzyme?

1. It recognizes a palindromic nucleotide sequence
2. It is an endonuclease
3. It is isolated from viruses
4. It produces the same kind of sticky ends in different DNA molecules

Q. Each restriction enzyme recognises a specific base sequence known as . This site is in sequence.

1. recognition site
2. recombinant site
3. palindromic
4. adaptive

Q.

Do eukaryotic cells have restriction endonucleases? Justify your answer.

Q.

What is the template-independent enzymatic synthesis of RNA?

Q.

Distance between two successive base pairs in DNA is:

1. 0.34 nm

2. 0.34 A

3. 3.4 nm

4. 34 A

Q.

Why are sticky ends better than blunt?

Q.

Who isolated the first restriction endonuclease?

Q.

Do restriction enzymes break hydrogen bonds?

Q. Regulation of gene expression in eukaryotes could be exerted at a. Transcriptional level (formation of primary transcript)
b Processing level (regulation of splicing)
c. Transport of mRNA from nucleus to the cytoplasm
d. Translational level

1. a, b and c
2. 
   a, b, c and d
3. 
   a, c and d
4. 
   b, c and d

Q.

What does the hybridization of DNA or RNA entail?

Q.

What is the anticodon for CCA?

Q. A conventional method for naming restriction enzymes is followed.In case of Eco RI, what do the following indicate

1. 1 - Strain 2 - Genus 3 - Order of isolation 4 - Species
2. 1 - Genus 2 - Order of isolation 3 - Strain 4 - Species
3. 1 - Genus 2 - Species 3 - Strain 4 - Order of isolation
4. 1 - Order of isolation 2 - Species 3 - Strain 4 - Genus

Q. How many types of restriction endonucleases are present.

1. Two types
2. Three types
3. Four types
4. Five types

Q. Which among the following are features required in a vector to facilitate cloning?
I. It should have ori - a sequence from where replication starts.
II. It should have a selectable marker.
III. It should have several recognition sites for a particular restriction enzyme.
IV. It should have a single recognition site for commonly used restriction enzymes.

1. I, II
2. I, II, III
3. I, III, IV
4. I, II, IV

Q. Restriction endonucleases are enzymes which

1. make cuts at specific positions within the DNA molecule
2. recognize a specific nucleotide sequence for binding of DNA ligase
3. restrict the action of the enzyme DNA polymerase
4. remove nucleotides from the ends of the DNA molecule

Q. Reverse transcriptase is also called

1. DNA dependent DNA polymerase
2. RNA dependent DNA polymerase
3. DNA dependent RNA polymerase
4. RNA dependent RNA polymerase

Q. Select the correct statement.
(a) RNA polymerase I transcribes rRNAs
(b) RNA polymerase II transcribes snRNA
(c) RNA polymerase lll transcribes hnRNA
(d) RNA polymerase II transcribes hnRNA

1. 
   b and c are correct
2. 
   a and d are correct
3. 
   a and c are correct.
4. 
   a and b are correct

Q. Which of the following tools of recombinant DNA technology is incorrectly paired with its use

1. EcoR I - production of sticky ends
2. DNA ligase - multiplication of rDNA molecules
3. DNA Polymerase - polymerase chain reaction (PCR) to amplify the DNA segments
4. Selectable marker - identification of transformants

Q. Sir do eukaryotes have restriction endonucleases?????
If no why ????

Q. Assertion: Traditional hybridisation leads to the inclusion and multiplication of undesirable along with the desired genes

Reason: Recombinant DNA technology allows insertion of the desired gene only into the target organisms

1. Both assertion and reason are true and the reason is a correct explanation of the assertion
2. Both assertion and reason are true but the reason is not a correct explanation of the assertion
   
3. Assertion is true but the reason is false
4. Both assertion and reason are false

Q.

What is Pulsed-field Gel Electrophoresis?

Q.

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

Q.

How is DNA inserted into a vector?

Q. What is the function of non-sense codon?

1. It translocate m-RNA to cytoplasm from DNA
2. Release the synthesized polypeptide chain from ribosomes
3. It carrying specific amino acid molecule on m-RNA
4. It release polypeptide chain from methionine
   

Q. The usual source of restriction endonucleases used in gene cloning is from

1. fungi
2. bacteria
3. plants
4. viruses