Sense and Antisense Strand

Q. What are sense , antisense , and mis sense strands ???

Q. If a double stranded DNA has 35% thymine, what would be the percentage of guanine in the DNA.

1. 35%
2. 70%
3. 30%
4. 15%

Q. Define cohesive end sites or cos sites

Q. The gene which codes for the proteins involved in the replication of the plasmid is termed:

1. Selectable marker
2. rop
3. ori
4. Antibiotic resistance

Q. What is the difference between template strand and coding strand

Q. The antisense strand of a fragment of DNA is AGGTATCCCAT. What will be the corresponding sequence of the antisense strand of mRNA?

1. UGGTUTCGCAT
2. AGGUAUCCCAU
3. ACCUAUGCGAU
4. UCCAUAGCGUA

Q. In a single-stranded circular DNA the percentage of Thymine is 15%. What will be the percentage of Adenine?

1. 15%
2. 30%
3. 45%
4. Cannot be detrmined

Q.

Antisense RNA is complementary to which of the following?

1. rRNA
2. tRNA
3. mRNA
4. snRNA

Q. The segment of master strand of DNA involved in transcription is called ?

1. Muton
2. Recon
3. Cistron
4. Sense strand

Q. In a segment of double stranded DNA 4.6 kilobases are present. If 630 adenine molecules are present, then what will be the number of cytosine molecules?

1. 630
2. 1260
3. 3340
4. 1670

Q. If a double-stranded DNA has 20% of cytosine, what will be the percentage of adenine in it?

1. 20%
2. 40%
3. 30%
4. 60%

Q. A strand of DNA has the following sequence: 5'-TACGTTACG-3'. What is the mRNA complement that will he generated from this DNA template through transcription?

1. 3`-ATGCAATGC-5'
2. 5`-ATGCAATGC-3'
3. 5'-AUGCAAUGC-3'
4. 3'-AUGCAAUGC-5'
5. 3`-ATTCAATGC-5'

Q. In a segment of double stranded DNA 4.6 kilobases are present. If 630 adenine molecules are present, then what will be the number of cytosine molecules?

1. 630
2. 1260
3. 3340
4. 1670

Q. Distance between two successive base pairs in DNA is

1. $0.34nm$
2. $0.34\mathring{A}$
3. $34\mathring{A}$
4. $3.4nm$

Q. How many of the following statements are correct?

A. Coding strand is also called a template strand which gets transcribed to RNA.

B. Template strand is considered as a non-coding strand which gets transcribed to RNA.

C. Coding strand of DNA (sense strand) has the same sequences as transcribed RNA, except for having thymine in place of RNA’s uracil.

D. The template strand of DNA has the same sequences as transcribed RNA.

E. Coding strand (sense strand) has the same sequences as transcribed RNA, except for having uracil in place of RNA’s thymine.

Q. Wilkins X-ray diffraction showed the diameter of the DNA helix is

1. 10 $\mathring{A}$
2. 20 $\mathring{A}$
3. 30 $\mathring{A}$
4. 40 $\mathring{A}$

Q. Write polarity of template strand and coding strand. Write three regions of transcription unit of DNA.

Q.

A DNA strand on which new strand is produced is called:

1. Complementary strand

2. Leading strand

3. Primer strand

4. Template strand

Q. If the sequence of bases in the coding strand of a double-stranded DNA is 5'-GTTCGAGTC-3' the sequence of bases in its transcript will be

1. 5'-GACUCGAAC-3'
2. 5'-CUGAGCUUG-3'
3. 5'-CAAGCUCAG-3'
4. 5'-GUUCGAGUC-3'

Q. The characteristic feature of Z-DNA is

1. Left handed
2. Right handed
3. 12 base pairs in each turn
4. Both A and C

Q. Which of the following is TRUE?

1. The leading strand in replication is the antisense strand in transcription
2. The lagging strand in replication is the template strand in transcription
3. None of the above
4. The lagging strand in replication is the sense strand in transcription

Q. The nucleotide sequence on antisense strand of DNA which is transcribed as codon that specifies the amino acid serine is

1. TAC
2. AGU
3. TCA
4. CCC

Q.

1. A and B are wrong but C and D are correct.
2. B is correct but A, C and D are wrong.
3. A, B and C are correct but D is wrong.
4. A and B are correct but C and D are wrong.
5. B, C and D are correct but A is wrong.

Q. If a segment of mRNA molecule has the sequence 5' GUACCGAUCG 3', which of the following could have been the template DNA molecule?

1. 5' GCUAGCCAUG 3'
2. 3' CATGGCTAGC 5'
3. 5' GUACCGAUCG 3'
4. 3' CGATCGGTAC 5'

Q. In a double stranded DNA which strand is transcribed. Why?

Q. Q.131. A 340 $\stackrel{o}{A}$ long segment of DNA molecule has 20 thymine nitrogenous bases, what will be the number of guanine nitrogen bases in the same segment?
A) 10
B) 40
C) 80
D) 160

Q.

Observe the given sequence of nitrogenous bases on a DNA fragment and

answer the following question –5´ – CAGAATTCTTA – 3´3´ – GTCTTAAGAAT – 5´(a) Name a restriction enzme which can recognise this DNA sequence.(b) Write the sequence after digestion.

Q. What will the RNA code if the DNA code is ATG?

1. AUG
2. ATG
3. UAC
4. TAC

Q. The two polynucleotide chains in a DNA are

1. Parallel
2. Discontinuous
3. Antiparallel
4. Semiconservative

Q. A DNA strand with the sequence $AACGTAACG$ is transcribed. What is the sequence of the mRNA molecule synthesized?

1. $AACGTAACG$
2. $UUGCAUUGC$
3. $AACGUAACG$
4. $TTGCATTGC$