Study of Physiology

Q. Agrobacterium tumefaciens: Gene transfer in plants :: Bacillus thuringiensis: _________

1. Herbicide
   
2. Pesticide
   
3. Biopesticide
   
4. Bio Fertilizer
   

Q. Select the odd one out w.r.t Gene therapy?

1. Agrobacterium
2. Retrovirus
3. Adenovirus
4. Herpes simplex virus

Q. Which of the following statements is not true about GM crops?

1. Enhanced nutritional value
2. GM crops are more susceptible to pests and abiotic stress
3. Increased efficiency of mineral usage by plants
4. GM crops helps in reducing post harvest loss

Q. Molecular scissors: Restriction endonucleases :: Molecular glue: _________

1. exonucleases
2. DNA ligase
3. RNA polymerase
4. restriction enzymes

Q.

Transgenic mice may be used for testing of ________________.

1. The safety of vaccines

2. Efficiency of fertilisers

3. Dose of antibiotics

4. All the above

Q. Assertion: A bacterial cell with restriction enzymes will be easily infected and lysed by bacteriophages.
Reason : Restriction enzymes catalyse synthesis of protective coat around bacterial cell that prevents the attack of bacteriophage.

1. Both assertion and reason are true and reason is the correct explanation of the assertion
2. Both assertion and reason are true but reason is not the correct explanation of the assertion
3. Assertion is true but reason is false
4. Both assertion and reason are false

Q. Bt-toxin works against which of the following order of insects?

1. Lepidopterans
2. Coleopterans
3. Dipterans
4. All of the above

Q. The first restriction endonuclease characterised was from the bacterium __i___ and was known as __ii__.

1. i- Escherichia coli, ii- EcoR I
2. i- Escherichia coli, ii- EcoR II
3. i- Haemophilus influenzae, ii- Hind II
4. i- Haemophilus influenzae, ii- Hind III

Q.

Who continued to fight a Gorilla war against British?

Q. Knife of DNA is:

1. DNA−ligase
2. Restriction enzyme
3. DNA polymerase
4. Peptidase

Q.

Which of the following is an advantage of transgenesis?

1. Study of diseases and gene therapy

2. Increased production of biological products

3. Vaccine safety testing

4. All of the these

Q. Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA is cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition site for the same restriction enzyme within the vector complicates gene cloning.

1. (i), (iii) and (v)
2. (ii) and (iv)
3. (iii), (iv) and (v)
4. (ii), (iii) and (iv)

Q. Identify the incorrect statement(s) regarding naming of restriction endonucleases.

1. The first letter in the name represents the family of the bacteria from which it was isolated
2. The second two letters represent genus of the bacteria
3. The Roman number in the name indicates the order in which it is discovered
4. All of the above

Q. Genetic Engineering Approval Committee (GEAC) is an Indian Government organisations for:

1. Giving patent for the GM plants
2. Protection of bio-resources and traditional knowledge
3. Fighting biopiracy in the country
4. Validiting GM research and the safety of introducing GM-organisms

Q.

Hirudin is

1. An antibiotic produced by a genetically engineered Escherichia coli

2. A lysine-rich protein produced by Hordeum vulgare

3. An anticoagulant protein that can be obtained from transgenic Brassica napus

4. A toxic molecule isolated from Gossypium hirsutum which reduces human fertility

Q. Study the following flowchart and identify the enzymes used in steps I and II.

1. EcoR I and Hind II respectively
2. EcoR I and DNA ligase
3. Hind II and DNA ligase
4. Restriction endonuclease and exonuclease

Q. Read the given statements and choose the correct options.
Statement 1 : Food derived from transgenic crops are called GM Foods.
Statement 2 : Health and food safety concerns have been raised to ensure the safety of GM foods

1. Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1
   
2. Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1
   
3. Statement 1 is correct but statement 2 is incorrect
   
4. Both statements 1 and 2 are incorrect

Q.

What is an example of a transgenic animal?

Q.

What can you infer from the following image?

1. The gene sequences from human chromosomes are found in Mice

2. The gene sequences of humans and mouse are completely different

3. The gene sequences of Mice are not found in humans

4. The functional genes are indicated on the mouse chromosomes

Q. Which of the following would be required for the synthesis of rDNA?
(I) Restriction endonuclease
(II) DNA fragments
(III) DNA ligase
(IV) DNA helicase

1. I, II, IV
2. I, II, III
3. I, III, IV
4. II, III, IV

Q. Which of the following crosses would produce a normal female progeny and a haemophilic female progeny in the F${{}_1}^{}$ generation?

1. Carrier female X normal male
2. Carrier female X haemophilic male
3. Normal female X haemophilic male
4. This condition is not possible in a single generation

Q. Read the following statements and from the options given below choose the correct one.

Statement 1: Deadly pathogens are used as bioweapons by the terrorists
Statement 2: Spores of Bacillus anthracis causes anthrax and was used as a bioweapon

1. Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1
2. Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1
3. Statement 1 is correct but statement 2 is incorrect
4. Both statements 1 and 2 are incorrect

Q. Full form of GMOs is:

1. Genetically Modern Organisms
2. Genetically Modified Organisms
3. Genetically Mutant Organisms
4. Genetically Male Organisms

Q. Which among the following is not a biocontrol agent?

1. Trichoderma
2. Nucleopolyhedrovirus (NPV)
3. Xanthomonas campestris
4. Bacillus thuringiensis

Q. Select the correct option for the given statements X, Y and Z.

1. Statements X and Y are wrong and statement Z is correct
2. Statements X, Y, Z are true and statement Z does not give correct explanation of X
3. Statements X, Y, Z are true and statement Z gives correct explanation for X
4. Statements X and Y are correct and statement Z is wrong

Q. Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA is cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition site for the same restriction enzyme within the vector complicates gene cloning.

1. (i), (iii) and (v)
2. (ii) and (iv)
3. (iii), (iv) and (v)
4. (ii), (iii) and (iv)

Q.

Which of the following holds true for smart mouse?

1. It has improved learning and memory

2. It can solve complex mathematical problems

3. The NR2B gene is knocked off

4. NR2B gene is over expressed

Q. Knife of DNA is:

1. DNA−ligase
2. Restriction enzyme
3. DNA polymerase
4. Peptidase

Q. Which of the following would be required for the synthesis of rDNA?
(I) Restriction endonuclease
(II) DNA fragments
(III) DNA ligase
(IV) DNA helicase

1. I, II, IV
2. I, II, III
3. I, III, IV
4. II, III, IV

Q. Name the first transgenic cow.