Ti Plasmids

Q.

What are Col-plasmids?

Q. The Ti plasmid, is often used for making transgenic plants. This plasmid is found in

1. Azotobacter
2. Rhizobium of the roots of leguminous plants
3. Agrobacterium
4. Yeast as a 2µm

Q. Read the following statements and choose the most appropriate option.
A. Agrobacterium tumefaciens is a pathogen of several monocot plants.
B. Exonucleases remove nucleotides from the ends of the DNA whereas endonucleases make cuts at specific positions within the DNA.

1. A is true and B is false
2. A is false and B is true
3. Both A and B are true
4. Both A and B are false

Q. Assertion :Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with roots of all cereal and pulse crops. Reason: A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop with which bacterium is associated.

1. If both Assertion and Reason are correct and Reason is the correct explanation of Assertion
2. If both Assertion and Reason are correct, but Reason is not the correct explanation of Assertion
3. If Assertion is correct but Reason is incorrect
4. If Assertion is incorrect but Reason is correct

Q. Ti plasmid that is used for introducing genes in plants is obtained from

1. Escherichia coli
2. Klebsiella
3. Agrobacterium tumefaciens
4. Agrobacterium rhizogenes

Q. Which is used for the introduction of alien DNA into the animal cell?

1. Agrobacterium tumefaciens
2. Retroviruses
3. Biolistics method
4. Both b and c

Q. Which of the following statements are incorrect with respect to mutations?
i. During the S phase of cell cycle, chances of mutation are higher.
ii. In spontaneous mutations, presence of mutagens is required.
iii. Cigarette compounds belong to the calss of physical mutagens.
iv. UV rays can cause dimerisation in the DNA.

1. i, ii, iii
2. ii, iii
3. iii, iv
4. i, iii

Q. The tumor-inducing capacity of Agrobacterium tumefaciensis located in large extra-chromosomal plasmids called ________.

1. Ri plasmid
2. Lambda phage
3. pBR322
4. Ti plasmid

Q. The introduction of genes into plant cells often makes use of -

Q. An example of a genetic engineer seen in nature is ________

1. Bacillus Subtilis
2. Pseudomonas sp
3. Escherichia coli
4. Agrobacterium Tumefaciens

Q.

How do plasmids contribute to antibiotic resistance?

Q.

This section contains 1 Assertion-Reason type question, which has 4 choices (a), (b), (c) and (d) out of which ONLY ONE is correct.

इस खण्ड में 1 कथन-कारण प्रकार का प्रश्न है, जिसमें 4 विकल्प (a), (b), (c) तथा (d) दिये गये हैं, जिनमें से केवल एक सही है।

A : Biotechnology can be used to produce transgenic bacteria that function as micro-factories for protein synthesis.

A : जैव प्रौद्योगिकी का उपयोग पारजीवी जीवाणु उत्पन्न करने के लिए किया जा सकता है जो प्रोटीन संश्लेषण के लिए सूक्ष्म फैक्ट्री के रूप में कार्य करता है।

R : Human protein α-1-antitrypsin has been produced by using transgenic animals, which is used to treat emphysema.

R : मानव प्रोटीन α-1-एंटीट्रिप्सिन को पारजीवी जंतुओं के उपयोग द्वारा उत्पन्न किया गया, जिसका उपयोग वातस्फीति के उपचार में किया जाता है।

1. Both (A) and (R) are true and (R) is the correct explanation of (A)
   
   (A) तथा (R) दोनों सही हैं तथा (R), (A) का सही स्पष्टीकरण है
2. Both (A) and (R) are true but (R) is not the correct explanation of (A)
   
   (A) तथा (R) दोनों सही हैं लेकिन (R), (A) का सही स्पष्टीकरण नहीं है
3. (A) is true but (R) is false
   
   (A) सही है लेकिन (R) गलत है
4. (A) is false but (R) is true
   
   (A) गलत है लेकिन (R) सही है

Q. A good vector in genetic engineering is

1. Agrobacterium tumefaciens
2. Bacillus thuringiensis
3. Bacillus amyloliquefaciens
4. Salmonella typhimurium

Q. Which of the following is correctly matched?

1. pBR322 – Enzyme
2. Agrobacterium tumefaciens – Tumour
3. Thermus aquaticus – Bt-gene
4. Ligase – Molecular scissors
5. Hind II – Plasmid vector

Q. Ti plasmid that is used for introducing genes in plants is obtained from

1. Escherichia coli
2. Agrobacterium tumefaciens
3. Agrobacterium rhizogenes
4. Klebsiella

Q. Assertion :The Ti plasmid of Agro bacterium tumefactions has been effectively used as a vector for gene transfer in plants. Reason: Ti plasmids carry sites for insertion of foreign gene intended to be transferred.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. A tumour inducing plasmid widely used in the production of transgenic plant is that of

1. Escherichia coli
2. Bacillus thuringiensis
3. Staphylococcus aureus
4. Agrobacterium tumefaciens

Q. Ti-plasmid used in genetic engineering has been modified by

1. Adding tumour forming genes
2. Adding genes for endonucleases
3. Deleting tumour forming genes
4. Deleting genes for endonucleases

Q. Two bacteria found to be very useful in genetic engineering experiment are

1. Nitrosomonas and Klebsiella
2. Escherichia and Agrobacterium
3. Nitrobacter and Azotobacter
4. Rhizobium and Diplococcus

Q. Why the production of a human protein in bacteria by genetic engineering became possible?

1. Bacterial cell can carry out the RNA splicing reactions
2. The human chromosome can replicate bacterial cell
3. The mechanism of gene regulation is identical in humans and bacteria
4. The genetic code is universal

Q. What is the source of Ti plasmid used in genetic engineering?

1. Escherichia coli
2. Bacillus thuringiensis
3. Agrobacterium tumefaciens
4. Agrobacterium thizogenes

Q. Choose the correct match.

1. Agrobacterium - T-DNA
2. Ligase - Molecular scissors
3. Hind II - Plasmid vector
4. Thermus - Restriction enzyme

Q. Which one of the following is commonly used in the transfer of foreign DNA into crop plants?

1. Trichoderma harzianum
2. Meloidogyne incognita
3. Agrobacterium tumefaciens
4. Penicillium expansum

Q. Which one of the following bacterium is used for production of transgenic plants?

1. Escherichia coli
2. Bacillus thuringiensis
3. Staphylococcus aureus
4. Agrobacterium tumefaciens

Q. The tumor inducing capacity of Agrobacterium tumefaciens, which is located in large extra - chromosomal plasmids called

1. III plasmid
2. Lambda phage
3. pBR 322
4. Ti plasmid

Q. Which one of the following microbes provides a natural system for gene transfer to produce transgenic plants?

1. Pseudomonas syringae
2. Agrobacterium tumefaciens
3. Bacillus thuringiensis
4. E. Coli

Q. A tumour including plasmid widely used in the production of transgenic plants is that of

1. Escherichia coli.
2. Bacillus thuringiensis.
3. Staphylococcus aureus.
4. Agrobacterium tumefaciens.

Q. In recombination process, which vector is used ?

1. Protein
2. Agrobacterium tumefaciens
3. Nucleic acid
4. Cellulose

Q. A bacteria that is helpful in creating transgenic plants is

1. Rhizobium
2. Agrobacterium
3. Pseudomonas
4. Azotobacter

Q. The tumor-inducing capacity of Agrobacterium tumefaciensis located in large extra-chromosomal plasmids called ________.

1. Ri plasmid
2. Lambda phage
3. pBR322
4. Ti plasmid