Tissue Culture

Q. Who is the father of Plant tissue culture?

1. Haberlandt
2. Gautheret
3. Steward
4. Bonner

Q.

Transplantation of tissues/organs to save certain patients often fails due to rejection of such tissues/organs in the patient’s body. Which type of immune response is responsible for such rejection? [1]

(a) Auto-immune response

(b) Humoral immune response

(c) Physiological immune response

(d) Cell-mediated immune response

Q.

What is Protein Assimilation?

Q. Embryo culture is used for________

1. establishing suspension culture
   
2. recovery of interspecific hybrids
   
3. somatic hybridization
   
4. haploid production
   

Q. Why is that the organs cannot be taken from just anybody during organ transplantation?

Q.

What is the site of vernalization ?

Q. State what is apomixis. Comment on its significance. How can it be commercially used? [2]

Q. Assertion (A): Tissue matching and blood group matching are essential before undergoing any organ transplantation.

Reason (R): Transplanted tissue has antigens which stimulate the specific immune response of the recipient.

1. Both A and R are true and R is the correct explanation of A
2. Both A and R are true but R is not the correct explanation of A
3. A is true but R is false
4. Both A and R are false

Q. A gene for corn has two alleles, one for yellow kernels and one for white kernels. Cross pollination of yellow corn and white corn results in ears of corn that have an approximately even mix of yellow and white kernels. Which term best describes the relationship between the two alleles?

1. Incomplete dominance
2. Codominance
3. Chimerism
4. Genetic recombination

Q.

Point out the difference between autogamy and allogamy.

Q. What is it that doctors check during organ transplantation?

Q.

Which among the following shows the correct chronological order of the events occurring during callus culture?

1. Callus – cell division – explant – cytokinin – acquire meristematic property

2. Callus – explant – cell division – cytokinin – cell acquire meristematic activity

3. Explant – callus – cell division – cytokinin – cell acquire meristematic activity

4. Explant – cell division – callus – cytokinin – cells acquire meristematic property

Q.

Are purple carrots GMO?

Q. TIBA is

1. an auxin
2. a kinetin
3. an antiauxin
4. a gibberellin

Q. Mention any two advantages of micropropagation technique. Write how its process is carried out in the laboratory. Name any two important food plants grown commercially by this method. [3 marks]

Q. Cell elongation in plants is caused by

1. auxin
2. gibberellin
3. florigen
4. traumatin

Q.

Explant required for virus free culture is

1. root

2. shoot tip

3. leaf

4. leaf and root

Q.

Which of the following factors control cell division and growth in plants?

1. Auxins and cytokinins

2. Auxins and, ethylene

3. Auxins and florigin

4. Auxins and abscisic acid

Q.

In crop improvement haploids are important because they

1. require one half of nutrients

2. are helpful in study of meiosis

3. pure lines can be formed quickly

4. give rise to hybrids very quickly

Q. In order to make virus-free banana, which method is used?

1. Meristem culture
2. Bioremediation
3. Somatic embryogenesis
4. Micro-propagation

Q. The technique of producing a large number of genetically similar plants within short time by tissue culture is called

1. Organogenesis
2. Protoplast culture
3. Micropropagation
4. Somatic hybridisation

Q. Which of the following step is the backbone of plant breeding programme?

1. Cross hybridization
2. Germplasm collection
3. Selection of superior hybrids
4. Selection of parents

Q. Assertion: Isolated protoplasts are used for somatic hybridisation
Reason: Callus culture does not allow variation

1. Both assertion and reason are true and reason is the correct explanation of assertion
2. Assertion is true but reason is false
3. Both assertion and reason are false
4. Both assertion and reason are true but reason is not the correct explanation of assertion

Q.

Name the Japanese cytologist who cultured anthers for the first time.

1. Shimakura

2. Yabuta

3. Sumuki

4. Hannig

Q.

Genetic engineering is a technique in which two plants with desired traits are made to cross and develop seeds.

1. True
2. False

Q.

Difference between vegetative propagation and Parenation?

Q. Auxins stimulate growth by means of

1. Cell maturation

2. Cell division

3. Cell elongation

4. Cell differentiation

Q. Read the following four statements (A-D).

1. Three
2. Four
3. One
4. Two

Q. The term tissue was coined by

1. Nageli
2. Virchow
3. Malpighi
4. Grew

Q. Match the following essential elements and their functions in plants.

(a)	Zn	(i)	Required for formation of mitotic spindle
(b)	Ca	(ii)	Constituent of biotin
(c)	S	(iii)	Constituent of nucleic acid
(d)	N	(iv)	Required for synthesis of auxin

Select the correct option.

1. (a) (b) (c) (d)
   (ii) (iii) (i) (iv)
2. (a) (b) (c) (d)
   (iii) (ii) (iv) (i)
3. (a) (b) (c) (d)
   (iv) (ii) (i) (iii)
4. (a) (b) (c) (d)
   (iv) (i) (ii) (iii)