pBR322

Q.

Plasmid pBR322 has a Pst l restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain:

1. It will lead to lysis of the host cell

2. It will not be able to confer ampicillin resistance to the host cell

3. It will be able to produce a novel protein with a dual ability

4. The transformed cells will have the ability to resist ampicillin as well as produce β-galactoside

Q.

Enumerate the importance of osmosis in the life of the plants.

Q.

How many selectable markers are present in pBR322?

Q. Polyethylene glycol method is used for

1. biodiesel production
2. seedless fruit production
3. energy production from sewage
4. gene transfer without a vector

Q.

Which cell organelle plays an important role during metamorphosis in frogs?

1. Mitochondria

2. Lysosome

3. Nucleus

4. Endoplasmic Reticulum

Q.

Mention one significance of osmosis

Q.

How do Ori and cloning sites facilitate cloning into a vector?

Q. The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?

1. amp${}^R$, tet${}^R$ - antibiotic resistance genes
2. rop - reduced osmotic pressure
3. Hind III, EcoRI - selectable markers
4. ori - original restriction enzyme

Q. Cutting of DNA at specific location by restriction enzymes is popularly called Molecular Scissors. The cut piece of DNA is linked with the plasmid DNA called

1. Vector
2. Invertor
3. Protractor
4. Reactor

Q.

What are selectable markers?

Q. Identify the labels A, B, C and D in the given diagram

1. A: EcoR I, B: BamH I, C: Ori, D: Ampicillin resistance gene
2. A: BamH I, B: EcoR I, C: Ampicillin resistance gene, D: Ori
3. A: origin of replication, B: Hind II, C: BamH I, D: amp^R
4. A: EcoR I, B: Hind II, C: Ori, D: amp^R

Q. During the process of isolation of DNA, chilled ethanol is added to

1. Facilitate action of restriction enzymes
2. Precipitate DNA
   
3. Break open the cell to release DNA
4. Remove proteins such as histones

Q.

What are $4$ major components found on a cloning vector?

Q. Which of the following(s) is/are selectable marker(s) in pBR322?

1. Methicillin resistance marker
2. Ampicillin resistance marker
3. Tetracycline resistance marker
4. Kanamycin resistance marker

Q.

What is the function of a selectable marker during transformation?

Q. Bolivar and Rodriguez synthesized an artificial ori site for pBR322 that helps in maintaining high copy number.

1. False
2. True

Q. Gel electrophoresis is used for

1. Separation of DNA fragments according to their size
2. Construction of recombinant DNA by joining with cloning vectors
3. Isolation of DNA molecules
4. Cutting of DNA into fragments

Q. Tryptophan operon of Escherichia coli is

1. Monocistronic
2. Unregulated
3. Repressible
4. Inducible

Q. Methyl guanosine triphosphate is added at 5’ end of hnRNA in a process of

1. None of the above
2. Capping
3. Splicing
4. Tailing

Q.

Define insertional inactivation.

Q. Methyl guanosine triphosphate is added at 5’ end of hnRNA in a process of

1. Capping
2. Tailing
3. Splicing
4. None of the above

Q. Bioreactors are useful in

1. isolation of genetic material
2. processing of large volume of culture
3. separation and purification of a product
4. micro-injection

Q.

Do plasmids carry essential genes?

Q. B and R in pBR322 indicates

1. Boyer and Ramsay
   
2. Bolivar and Rodriguez
3. Bohr and Ray
4. Bacon and Raman

Q. Identify the plasmid

1. EcoRI
2. pBR 322
3. AIUI
4. Hind III

Q. Match the options of column I with the correct alternative in column II.

	Column I		Column II
P	Restriction enzymes	1	Tumour inducing plasmid
Q	Agrobacterium tumefaciens	2	Cleaves DNA at specific sites
R	Kary Mullis	3	Insecticidal protein
S	Bt	4	Polymerase Chain Reaction

1. P-2, Q-1, R-4, S-3
2. P-1, Q-2, R-4, S-3
3. P-2, Q-1, R-3, S-4
4. P-1, Q-2, R-3, S-4

Q. Genetic code was discovered by

1. Holley and Nirenberg
2. Nirenberg and Matthei
3. Holley Nirenberg and Khorana
4. W. Fleming and Matthei

Q. Identify the plasmid.

1. pBR322
2. Eco RI
3. Hind II
4. Hind III

Q.

Water passes into a root by the process called _______.

Q. What does pBR322 stand for?

1. Plasmid bearing 322 restriction sites
2. Plasmid made by Boyer and Rodriguez.
3. Plasmid made by Bolivar and Rodriguez
4. Plasmid containing just 322 nucleotides.