pSC 101

Q. The first plasmid vector used in genetic engineering was

1. pUC vectors
2. pSC 101
3. pUC 19
4. pBR322

Q. Which of the following is not a characteristic of pBR322 vector?

1. It is the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez
2. It is the most widely used, versatile and easily manipulated vector
3. It has two antibiotic resistance genes, $te{t}^R$ and $am{p}^R$
4. It does not have restriction site for Sal I

Q. Match the organism with its use in biotechnology.

(a)	Bacillus thuringiensis	(i)	Host cell for human insulin production
(b)	Thermus aquaticus	(ii)	Taq polymerase
(c)	Agrobacterium tumefaciens	(iii)	Insecticidal protein coding gene
(d)	E. coli	(iv)	Ti plasmid

Select the correct option from the following.

1. (a) (b) (c) (d)
   (iii) (ii) (iv) (i)
2. (a) (b) (c) (d)
   (iii) (ii) (i) (iv)
3. (a) (b) (c) (d)
   (ii) (iii) (i) (iv)
4. (a) (b) (c) (d)
   (i) (ii) (iii) (iv)

Q.

who discovered plasmid?

Q. Which of the following statement is/are incorrect regarding the plasmid pSC101?

i. It had one EcoR1 restriction site
ii. It had a tetracycline resistant gene
iii. Restriction site for EcoR1 was located in tetracycline gene
iv. It had kanamycin resistant gene

1. i
2. vi
3. ii and iii
4. iii and iv

Q. Match the organism with its use in biotechnology.

(a)	Bacillus thuringiensis	(i)	Host cell for human insulin production
(b)	Thermus aquaticus	(ii)	Taq polymerase
(c)	Agrobacterium tumefaciens	(iii)	Insecticidal protein coding gene
(d)	E. coli	(iv)	Ti plasmid

Select the correct option from the following.

1. (a) (b) (c) (d)
   (iii) (ii) (iv) (i)
2. (a) (b) (c) (d)
   (iii) (ii) (i) (iv)
3. (a) (b) (c) (d)
   (ii) (iii) (i) (iv)
4. (a) (b) (c) (d)
   (i) (ii) (iii) (iv)

Q. Gibberellin was first isolated from

1. A bacterium
2. A fungus
3. An alga
4. A virus

Q. Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

1. $\lambda$ phage
2. Retrovirus
3. PBR 322
4. Ti plasmid

Q.

The antibiotic commonly used in inducing the mutations in cytoplasmic genes is

1. Tetramycin
2. Streptomycin
3. Cyclosporin
4. Tetracycline

Q. The plasmid used by Cohen and Boyer for the first transformation experiment was

1. pSC 101
2. PUC 17
3. pBR 322
4. pBR 400

Q.

why does the antibiotic resistance gene loses its tetracycline resistance when foreign dna attaches?