Extraction of Genomic DNA
Trending Questions
Q. When using split pea for DNA extraction, chitinase can be used for lysing the cell wall of the pea cells.
- True
- False
Q. Arrange the steps for identifying the gene of interest:
- Southern Blot
- Electrophoresis
- Cut DNA
- Elution
Q. Histone is a protein that primarily works as a DNA packaging molecule.
- True
- False
Q. Detergent is used to break open the cell membrane. How does detergent aid in this process?
- Detergent dissolves the cell membrane because of its alkalinity
- Cell membrane reacts with the acidic detergent and is dissolved in it
- Detergent breaks the cell membrane after binding to the hydrophobic lipid tails
- Detergent can make holes in the cell membrane for the DNA to come out
Q. is used to break down the cell membrane of a cell.
- DNA ligase
- Detergent
- Cellulase
- Polymerase
Q. Match the following cells to their enzymes used for rupturing cells:
- Lysozyme
- Cellulase
- Chitinase
Q. First step in genetic engineering is
- Isolation of RNA
- Isolation of protein
- Isolation of genetic material
- Purification of protein
Q. If proteases are not used during DNA extraction, what would be the end result?
- DNA would be cut into small pieces by nucleases
- Helicase can unwind the DNA once the cell is ruptured
- DNA can bind to certain proteins once it is out of nuclear membrane and hence they need to be destroyed
- DNA is bound with histone proteins that needs to be digested