Obtaining Desired Gene

Q. Some of the steps involved in the production of human insulin are given below. Choose the correct sequence
i. Obtaining of gene for human insulin using c-DNA technology
ii. Culturing recombinant E-coli in bioreactors
iii. Purification of human insulin
iv. Insertion of human insulin gene into plasmid
v. Introduction of recombinant plasmid into E.coli
vi. Extraction of recombinant gene product from E-coli

1. ii-i-iv-iii-v-vi
2. i-iii-v-vi-ii-iv
3. i-iv-v-ii-vi-iii
4. iii-v-ii-i-vi-iv

Q. The main challenge for the production of insulin using rDNA technique was

1. Joining chain A with C peptide chain
2. Getting insulin assembled into its mature form
3. Removing B peptide chain
4. Separating chain A and chain B

Q.

Human insulin is being commercially produced from a transgenic species:

1. Rhizobium

2. Saccharomyces

3. Escherichia

4. Mycobacterium

Q. Transcribed regions present in mature mRNA arise from ---(i)-- and transcribed regions not present in mature mRNA arise from ----ii----.

1. i. intron, ii. exon
2. i. exon, ii. intron
3. i. codon, ii. anticodon
4. i. anticodon, ii. codon

Q. The main challenge for the production of insulin using rDNA technique was

1. Joining chain A with C peptide chain
2. Getting insulin assembled into its mature form
3. Removing B peptide chain
4. Separating chain A and chain B