Palindrome Sequences

Q. Which of the following is a palindromic sequence?

1. 5’-CGTATG-3’
   3’-GCATAC-5'
2. 5’-GAATTC-3’
   3’-CTTAAG-5’
3. 5’-GACTAC-3’
   3’-TACGAC-5'
4. 5’-CGAATG-3’
   3’-CGAATG-5’

Q. Identify the incorrect statement given below.

1. Each restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA
2. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strand
3. The ends cut by restriction enzymes can be joined together (end-to-end) using DNA ligases
4. Endonucleases remove nucleotides from the ends of the DNA whereas, exonucleases make cuts at specific positions within the DNA

Q. Which one of the following is a palindromic base sequence in DNA that can be easily cut at at the middle by some particular restriction enzyme?

1. 5’-------------CATTCG--------------------3’
   3’-------------GTAAGC-------------------5’
2. 5’------------GATATG------------------3’
   3’------------CTATAC-------------------5’
3. 5’------------GAATTC-------------------3’
   3’-------------CTTAAG-------------------5’
4. 5’-------------CACGTA------------------3’
   3’-------------CTGCAT------------------5’

Q. From the given options, identify the palindromic sequence containing 5’ GTT sequence.

1. 5^’ GTTAAC 3^’
   3^’ CAATTG 5^’
2. 5^’ GTTCCA 3^’
   3^’CAAGGT 5^’
3. 5^’ GAATTC 3^’
   3^’ CTTAAG 5^’
4. 5^’ CAGTAA 3^’
   3^’ GTCATT 5^’

Q. Which of the following is a palindromic sequence?

1. 5’-CGTATG-3’
   3’-GCATAC-5'
2. 5’-CGAATG-3’
   3’-CGAATG-5’
3. 5’-GAATTC-3’
   3’-CTTAAG-5’
4. 5’-GACTAC-3’
   3’-TACGAC-5'

Q. Which of the following DNA sequences qualifies to be designated as a palindrome?

1. 5’ – GACCAG – 3’ in one strand
2. 3’ – GACCAG – 5’ in one strand
3. 5’ – GACGAC = 3’ , 3’ – CGGGTC – 5’
4. 5’ – AGCGCT – 3’ , 3’ – TCGCGA – 5’