Recombinant DNA Technology

Q.

What are the three methods of genetic transfer in bacteria?

Q.

Brief about the method involved in gene cloning.

Q. Bacteria having rDNA containing both tetracycline and kanamycin resistant genes will survive in media with:

1. Tetracylcine only
2. Kanamycin only
3. Any antibiotics
4. Both kanamycin and tetracycline

Q. It is theoretically possible to transfer any gene from one species to another species through rDNA technology.

1. False
2. True

Q. Who was the pioneer of recombinant DNA (rDNA) technology?

1. Har Gobind Khorana
2. James D Watson
3. Stanley Cohen
4. Walter Sutton

Q. Bacteria having rDNA containing both tetracycline and kanamycin resistant genes will survive in media with:

1. Tetracylcine only
2. Kanamycin only
3. Any antibiotics
4. Both kanamycin and tetracycline

Q.

What is true about recombinant DNA technology?

1. Antibiotic-resistant genes can be inserted into a bacteria using r-DNA technology.

2. Foreign gene will always multiply when inserted in host DNA
   

3. There is no use of DNA ligase

4. All of the above

Q. Given below are a few diagrams with each representing a step in rDNA technology. Match the diagrams with the steps they represent.

i. Cloning
ii. Screening
iii. Gene incorporated in a vector
iv. Inserted into a host
v. Multiple copies using PCR
vi. Isolation of gene of interest

1. $p\Rightarrow iv.,q\Rightarrow v.,r\Rightarrow v.,s\Rightarrow v.,t\Rightarrow ii.,u\Rightarrow i$
2. $p\Rightarrow iii.,q\Rightarrow iv.,r\Rightarrow vi.,s\Rightarrow v.,t\Rightarrow ii.,u\Rightarrow i$
3. $p\Rightarrow ii.,q\Rightarrow iii.,r\Rightarrow v.,s\Rightarrow v.,t\Rightarrow iv.,u\Rightarrow i$
4. $p\Rightarrow v.,q\Rightarrow vi.,r\Rightarrow i.,s\Rightarrow ii.,t\Rightarrow iii.,u\Rightarrow iv$

Q. Sort the stepwise processes in rDNA technology.

1. Identify GOI
2. Copies of GOI
3. Insertion of GOI
4. Screening
5. Cloning

Q. The technique used in biotechnology is

1. hybridization
2. Recombinant DNA technology
3. Both a & b
4. None of these

Q. technique makes it possible to genetically engineer living organisms.

1. Recombinant DNA
2. X-ray diffraction
3. Isotope labeling
4. Hybridization

Q. Which one of the following techniques made it possible to genetically engineer living organisms?

1. Recombinant DNA techniques
2. X-ray diffraction
3. Heavier isotope labeling
4. Hybridization

Q. With regards to DNA extraction which of the following is/are correct?

1. Only the purest DNA samples can be used in rDNA technology.
2. DNA is present inside the nucleus of the cell which is embedded in the cytoplasm along with many organelles.
3. DNA extraction increases the success of recombination.
4. Since DNA extraction is easy, the step need not be avoided.