Screening of Recombinant Cell
Trending Questions
Q. Which of the following is correct in the process of insertional inactivation?
- A recombinant DNA is inserted within the recognition site for EcoRI
- A recombinant DNA is inserted within the coding sequence of enzyme β-galactosidase
- A recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid
- The coding sequence of enzyme β-galactosidase is inserted in the recombinant DNA
Q. Which of the following is correct in the process of insertional inactivation?
- A recombinant DNA is inserted within the recognition site for EcoRI
- A recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid
- The coding sequence of enzyme β-galactosidase is inserted in the recombinant DNA
- A recombinant DNA is inserted within the coding sequence of enzyme β-galactosidase
Q. Read the given statements and select the correct option.
Statement-1: In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement-2: Presence of insert results into insertional inactivation of beta- galactosidase enzyme and the colonies do not produce any colour.
Statement-1: In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement-2: Presence of insert results into insertional inactivation of beta- galactosidase enzyme and the colonies do not produce any colour.
- Both statements 1 and 2 are correct and statement 2 is correct explanation of statement 1
- Both statements 1 and 2 are correct but statement 2 is not correct explanation of statement 1
- Statement 1 is correct and statement 2 is incorrect
- Both statements 1 and 2 are incorrect
Q.
Which of the following is true about insertional inactivation of the β-galactosidase coding sequence?
The transformants exhibit blue coloured
The non-transformants exhibit blue coloured
The transformants do not exhibit any colour
The Non-transformants do not exhibit any colour