Screening of Recombinant Cell

Q. Which of the following is correct in the process of insertional inactivation?

1. A recombinant DNA is inserted within the recognition site for EcoRI
2. A recombinant DNA is inserted within the coding sequence of enzyme β-galactosidase
3. A recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid
4. The coding sequence of enzyme β-galactosidase is inserted in the recombinant DNA

Q. Which of the following is correct in the process of insertional inactivation?

1. A recombinant DNA is inserted within the recognition site for EcoRI
2. A recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid
3. The coding sequence of enzyme β-galactosidase is inserted in the recombinant DNA
4. A recombinant DNA is inserted within the coding sequence of enzyme β-galactosidase

Q. Read the given statements and select the correct option.
Statement-1: In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement-2: Presence of insert results into insertional inactivation of beta- galactosidase enzyme and the colonies do not produce any colour.

1. Both statements 1 and 2 are correct and statement 2 is correct explanation of statement 1
2. Both statements 1 and 2 are correct but statement 2 is not correct explanation of statement 1
3. Statement 1 is correct and statement 2 is incorrect
4. Both statements 1 and 2 are incorrect

Q.

Which of the following is true about insertional inactivation of the β-galactosidase coding sequence?

1. The transformants exhibit blue coloured

2. The non-transformants exhibit blue coloured

3. The transformants do not exhibit any colour

4. The Non-transformants do not exhibit any colour