Selection Markers

Q.

What are selectable markers? Give two examples.

Q.

What are antibiotic resistance genes?

Q. Which of the following helps in screening for recombinant cells?

1. Selection Marker such as antibiotic resistant gene
2. A gene in plasmid that produced human protein
3. A gene that would convert the glucose in the media to fructose
4. All of the above

Q. The given figure is the diagrammatic representation of the E. Coli vector pBR 322. Which one of the given options correctly identifies its certain components(s)?

1. Ori – original restriction enzyme
2. Rop – reduced osmotic pressure
3. Hin d III, Eco R I – selectable markers
4. amp, tet - Antibiotic resistance genes

Q. Regarding Assertion and Reason, choose the correct option.
Assertion[A]: Selection markers give straightforward results as to which cells have been transformed.
Reason[R]: Screening markers need X-gal stain to visualize the result of transformation procedure.

1. [A] is true and [R] is false.
2. [A] is false and [R] is true .
3. Both [A] and [R] are true and [R] is a correct explanation to [A].
4. Both [A] and [R] are true but [R] is not a correct explanation to [A].