Selection of Transformed Cell

Q. Which of the following help in identifying and eliminating non-transformants and selectively permitting the growth of the transformants?

1. Selectable markers
2. Ori sites
3. Structural genes
4. Recognition sites

Q.

How many base pairs is pUC18?

Q. Which of the following help in identifying and eliminating non-transformants and selectively permitting the growth of the transformants?

1. Selectable markers
2. Structural genes
3. Recognition sites
4. Ori sites

Q. In blue/white screening, the transformed cells with rDNA will exhibit

1. blue colour
2. white colour
3. blue and white colour
4. no colonies

Q. Which of the following parts of the plasmid can help in selection of transformed organism?

1. Replicon
2. Ori site
3. Antibiotic resistance gene
4. Gene of interest

Q. Which of the following parts of the plasmid can help in selection of transformed organism?

1. Gene of interest
2. Replicon
3. Antibiotic resistance gene
4. Ori site

Q. Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plated on two different media - one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector

1. will grow on both tetracycline containing and ampicillin containing media
2. will not grow on either tetracycline containing or ampicillin containing media
3. will not grow on tetracycline, but not on ampicillin-containing media
4. will grow on ampicillin but not on tetrecycline-containing media