Ti Plasmid

Q.

Bacillus thuringiensis is used as

1. Biofungicide

2. Biopesticide

3. Biocontroller

4. Bioweapon

Q. Which of the following represents the correct sequence of steps in leaf disc transfer method?

1. Dedifferentiation $\to$ cutting leaf discs $\to$ redifferentiation $\to$ agrobacterium infection
2. Cutting leaf discs $\to$ dedifferentiation $\to$ agrobacterium infection $\to$ redifferentiation
3. Agrobacterium infection $\to$ redifferentiation $\to$ cutting leaf discs $\to$ dedifferentiation
4. Cutting plant discs $\to$ agrobacterium infection $\to$ dedifferentiation $\to$ redifferentiation

Q. Wild Ti plasmid is able to induce tumors in plants due to the presence of:

1. Cancer genes
2. Phytohormone coding genes
3. Kanamycin resistance gene
4. Cry genes

Q. Which of the following modifications are made to make a “tamed” Ti plasmid which can transfer cry genes to a plant’s genome?

1. Removal of kanamycin resistance gene and adding phytohormone genes
2. Removal of phytohormone gene and adding kanamycin resistance gene
3. Removal of both kanamycin resistance gene and phytohormone genes
4. Adding both phytohormone and kanamycin resistance gene

Q. Agrobacterium naturally inserts DNA into plants via the

1. Ti plasmid
2. Cosmid
3. Marker gene
4. Antibiotic resistant gene