Ti Plasmids

Q. The introduction of t-DNA into plants involves

1. Allowing the plant roots to stand in water
2. Infection of the plant by Agrobacterium tumefaciens
3. Altering the pH of the soil, then heat shocking the plants
4. Exposing the plants to cold for a brief period

Q. A bacteria that is helpful in creating transgenic plants is

1. Rhizobium
2. Agrobacterium
3. Pseudomonas
4. Azotobacter

Q. Which one of the following is commonly used in the transfer of foreign DNA into crop plants?

1. Trichoderma harzianum
2. Meloidogyne incognita
3. Agrobacterium tumefaciens
4. Penicillium expansum

Q. The tumor-inducing capacity of Agrobacterium tumefaciensis located in large extra-chromosomal plasmids called ________.

1. Ri plasmid
2. Lambda phage
3. pBR322
4. Ti plasmid

Q. Ti plasmid that is used for introducing genes in plants is obtained from

1. Escherichia coli
2. Agrobacterium tumefaciens
3. Agrobacterium rhizogenes
4. Klebsiella

Q. An example of a genetic engineer seen in nature is ________

1. Bacillus Subtilis
2. Pseudomonas sp.
3. Escherichia coli
4. Agrobacterium tumefaciens

Q. Regarding assertion and reason, select the correct option
Assertion: Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops.
Reason: A gene incorporated in the bacterial chromosomal gene gets automatically transferred to the crop with which the bacterium is associated.

1. Both the assertion and reason are true and the reason is the correct explanation for the assertion.
2. Both the assertion and reason are true but the assertion is not the correct explanation of the reason.
3. Assertion is true but the reason is false
4. Both the assertion and reason are false.

Q. The tumor-inducing capacity of Agrobacterium tumefaciensis located in large extra-chromosomal plasmids called ________.

1. Ri plasmid
2. Lambda phage
3. Ti plasmid
4. pBR322