Transformation

Q. In which of the following methods, gold particles are used to introduce alien DNA into host cells?

1. Micro-injection
2. Biolistics
3. Gene gun
4. Both b and c

Q. Regarding Assertion and Reason, select the right option
Assertion [A]: $CaC{l}_2$ is necessary to create heat-shock competent cells.
Reason [R]: $CaC{l}_2$ imparts a positive charge to the cell.

1. [A] is true and [R] is false.
2. [A] is false and [R] is true .
3. Both [A] and [R] are true and [R] is a correct explanation to [A].
4. [Both [A] and [R] are true but [R] is not a correct explanation to [A].

Q. A cell is said to be competent when it is ready to accept a foreign DNA molecule.

1. True
2. False

Q. A cell is said to be competent when it is ready to accept a foreign DNA molecule.

1. True
2. False

Q. The correct sequence of steps for making a cell competent is:

I. Treatment with divalent cations
II. Heat shock ( $42°$C)
III. Placing on ice
IV. Incubation of cells with rDNA on ice

1. I → IV → II → III
2. II → IV → I → III
3. I → III → IV → II
4. IV → II → I → III

Q. Transformation, where plasmids enter bacterial cells, does not occur in nature.

1. True
2. False