What is PCR

Q.

What are four important PCR applications?

Q.

What is microsatellite instability?

Q. Why does PCR amplify DNA strands so rapidly?

1. PCR causes DNA bases to multiply
2. PCR includes oxidation of DNA
3. PCR is an addition reaction
4. PCR is an exponential reaction

Q. PCR amplifies DNA strands in an exponential rate.

1. False
2. True

Q.

Which of the following scientists won the Nobel Prize for the development of polymerase chain reaction (PCR) in the year 1993?

1. Caesar and Milstein

2. Philip Sharp

3. Susumu Tonegawa

4. Kary Mullis

Q. Which of the follwoing is true regarding the DNA polymerase used in PCR?

1. It is used to ligate introduced DNA in recipient cells.
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperature

Q. The purpose of polymerase chain reaction is __________

1. DNA modification
2. DNA amplification
3. DNA replication
4. DNA visualization

Q. Which of the following(s) is/are reqiured in PCR?

1. Nucleotides
2. Buffer
3. DNA polymerase
4. Primer

Q. Which of the following(s) is/are reqiured in PCR?

1. Nucleotides
2. Buffer
3. DNA polymerase
4. Primer

Q. Which of the following polymerases are used in PCR?

1. DNA polymerase I
2. DNA polymerase I
3. DNA polymerase III
4. Taq polymerase

Q. What is the main reason for PCR to be employed in rDNA technology?

1. DNA isolated from a cell sample will always be very less
2. Sometimes the DNA extraction from certain cells is very tough
3. PCR increase the chance of DNA isolation
4. PCR helps in identifying the gene of interest very easily