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Question
α−amylase activity test in barley endosperm is bioassay of :
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Solution
The correct option is D Gibberellin
A bioassay is described that gibberellin induced α−amylase release from barley half-seeds is proportional to the logarithm of gibberellin concentration.
Bioassay of gibberellin is performed through dwarf pea test, dwarf corn test and barley endosperm bioassay. Gibberellins were first discovered in Japan from a fungus Gibberella fujikuroi. They can stimulate stem elongation and dwarfism can be restored by spraying gibberellins.
Gibberellins are involved in seed germination in certain plants. In a classic experiment involving barley seed germination, researchers showed that the release of gibberellin from the embryo triggers the synthesis of a-amylase, an enzyme that digests starch in the endosperm.
As a result, glucose becomes available for absorption by the embryo. Although enzymes mobilize starch reserves in many types of seeds, gibberellin control of seed enzymes appears restricted to cereals and other grasses. In addition to mobilizing food reserves in newly germinated grass seeds, application of gibberellins substitutes for low-temperature or light requirements for germination in seeds of plants such as lettuce, oats and tobacco.
Thus, the correct answer is option D.
A bioassay is described that gibberellin induced α−amylase release from barley half-seeds is proportional to the logarithm of gibberellin concentration.
Bioassay of gibberellin is performed through dwarf pea test, dwarf corn test and barley endosperm bioassay. Gibberellins were first discovered in Japan from a fungus Gibberella fujikuroi. They can stimulate stem elongation and dwarfism can be restored by spraying gibberellins.
Gibberellins are involved in seed germination in certain plants. In a classic experiment involving barley seed germination, researchers showed that the release of gibberellin from the embryo triggers the synthesis of a-amylase, an enzyme that digests starch in the endosperm.
As a result, glucose becomes available for absorption by the embryo. Although enzymes mobilize starch reserves in many types of seeds, gibberellin control of seed enzymes appears restricted to cereals and other grasses. In addition to mobilizing food reserves in newly germinated grass seeds, application of gibberellins substitutes for low-temperature or light requirements for germination in seeds of plants such as lettuce, oats and tobacco.
Thus, the correct answer is option D.
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