The correct option is B the removal of PO3−4 ions
PO3−4 removal columns are designed to remove PO3−4 from a buffer solution. For example, the PO3−4 concentration can be reduced from 10mM to lower than 0.001mM. The principle of phosphate removal is based on interactions between PO3−4 and column resin. The PO3−4 stays on the column. Other buffer components including regular buffer salts and non-charged molecules such as sugar and glycerol do not bind to the resin, therefore they stay in the sample solution.