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Standard XII

Biology

PCR in Diagnosis

A certain pur...

Question

A certain purified DNA was cut with two restriction endonucleases E1 and E2. The results were obtained from agarose gel electrophoresis
Sample cut with E1 alone: two bands of size 35 kb and 15 kb
Sample cut with E2 alone: two bands of size 40 kb and 10 kb
Sample cut with E1 and E2 simultaneously: three bands of size 35 kb, 10kb and 5 kb
From this data it can be inferred that the DNA has

2 sites for E1 and 1 site for E2

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1 site for E1 and 2 site for E2

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1 site each for E1 and E2

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3 sites for E1 and 1 site for E2

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Solution

The correct option is C 1 site each for E1 and E2
The total weight of DNA fragment is 50 kb
Enzyme E1 cuts at 35 kb and enzyme E2 cuts DNA at 40kb.

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Similar questions

Q. A certain purified DNA sample was cut with two restriction endonucleases

E 1

and

E 2

. The following results were obtained from agarose gel electrophoresis
Sample cut with

E 1

alone: two bands of size

35 k b

and

15 k b

Sample cut with

E 2

alone: two bands of size

40 k b

and

10 k b

Sample cut simultaneously with

E 1

and

E 2

: three bands of size

35 k b, 10 k b

and

5 k b

From these data, it can be inferred that the DNA has

A certain purified DNA was cut with two restriction endonucleases E1 and E2. The results were obtained from agarose gel electrophoresis

Sample cut with E1 alone: two bands of size 35 kb and 15 kb

Sample cut with E2 alone: two bands of size 40 kb and 10 kb

Sample cut with E1 and E2 simultaneously: three bands of size 35 kb, 10kb and 5 kb

From this data it can be inferred that the DNA has

$A \to A^{+} e, E_{1} a n d A^{+} \to A^{2 +} + e, E_{2}$ .
1. The energy required to pull out the two electrons are $E_{1}$ and $E_{2}$ respectively. The correct relationship between two energy would be

Q. For any two independent events

E_{1}

and

E_{2}, P {(E_{1} \cup E_{2}) \cap (_{1}) \cap (_{2})}

Q. A circular double-stranded DNA molecule of 45kb size has two restriction sites for EcoRⅠ and one for HindⅢ. All of them are equidistant from each other. Upon incubation with both the restriction enzymes _____ band(s) appear in the gel electrophoresis.

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The correct option is C 1 site each for E1 and E2The total weight of DNA fragment is 50 kb Enzyme E1 cuts at 35 kb and enzyme E2 cuts DNA at 40kb.

The correct option is C 1 site each for E1 and E2
The total weight of DNA fragment is 50 kb
Enzyme E1 cuts at 35 kb and enzyme E2 cuts DNA at 40kb.