Question

A certain tissue of a plant infected with TMV was used to obtain a new plant using tissue culture technique. Identify the technique used and reason the possibility of obtaining a new healthy plant.

Answer 1

The tissue used for obtaining the new plants using tissue culture method is meristems. As we know that the meristems are not affected by virus infection thus they are safe to serve as explant material for the process. The tissue meristem was used as it is a totipotent tissue (Undifferentiated tissue capable of giving rise to an entire organism) and can give rise to all the tissues present in a mature plant.