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Enzyme Catalysis

A circular pl...

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A circular plasmid of 10, 000 base pairs (bp) is digested with two restriction enzymes A & B, to produce a 3000 BP & 2000 bp bands when visualised on an agarose gel. When digested with one enzymes at a time, only one band is visible at 5000 bp. If the first site for enzyme A(A1) is present at the 100th base, in order in which the remaining sites (A2, B1, B2) are present in it A) 3100,5100,8100 B) 8100 3100,5100 C) 5100,3100,8100 D) 8100,5100,3100

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Q. A circular plasmid of

10, 000

base pairs (bp) is digested with two restriction enzymes, A and B, to produce a

3000

2000

bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at

5000

bp. If the first site for enzyme A(

A 1

) is present at the

100^{t h}

base, the order in which the remaining sites (

A 2, B 1

B 2

) are present is?

Q. Recognition site plasmid having 2 Kbp at 500 bp, 800 bp, 1300 bp, 2000 bp
How many bands are visualise after gel electrophoresis after treating with R.E.

Q. As indicated in the gel image, lanes X and Y represent samples obtained from a circular plasmid DNA after complete digestion using restriction enzyme X or Y with different recognition sites, respectively. How many sites for X and Y are present in the plasmid (sizes of the bands in kilo base pairs(kb) is shown)?

Q. How many of the following statements are correct?
I. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites, 4 fragments would be produced.
II. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 6 fragments would be produced.
III. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites on the DNA, 5 fragments would be produced.
IV. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 7 fragments would be produced.

Q. How many linear DNA fragments will be produced when a circular plasmid is digested with a restriction enzyme having 3 sites?

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