Question

A combination of paper chromatography and electrophoresis involves :

• A. partition chromatography
• B. electrical mobility of the ionic species
• C. both (a) and (b)
• D. none of these

Answer 1

The correct option is C both (a) and (b)

Capillary electrochromatography is a combination of two analytical techniques, High performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis aims to separate analytes on the basis of their mass to charge ratio by passing a high voltage across ends of a capillary tube, which is filled with the analyte. High performance liquid chromatography separates analytes by passing them, under high pressure, through a column filled with stationary phase. Although Capillary Zone Electrophoresis (CZE) is a high-efficiency separation technique for charged analytes, it is incapable in its native form of separating neutral molecules.