A DNA sequencing reaction was performed with the fragment $5 - X X X G C G A T C G Y Y Y Y - 3^{'}$ as the template, dideoxy GTP, all the four $d N T P s$ , and the required primers and enzyme. $X X X X$ and $Y Y Y Y$ in the given DNA fragment represent primer binding sites. The set of fragments obtained during the reaction will be (the primers are not shown in the amplified fragments).

5^{'} - C G A T C G C - 3^{'}

only

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5^{'} - C G - 3^{'}, 5^{'} - C G C T A G - 3^{'}, 5^{'} - C C C T A G C - 3^{'}

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5^{'} - C G - 3^{'}, 5^{'} - C G A T C G - 3^{'}, 5^{'} - C G A T C G C - 3^{'}

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5^{'} - G - 3^{'}, 5^{'} - G C G - 3^{'}, 5^{'} - G C G A T C G - 3^{'}

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Solution

The correct option is A $5^{'} - C G A T C G C - 3^{'}$ only
A DNA sequencing reaction was performed with the fragment 5−XXXGCGATCGYYYY−3′ as the template, dideoxy GTP, all the four dNTPs, and the required primers and enzyme. XXXX and YYYY in the given DNA fragment represent primer binding sites. The set of fragments obtained during the reaction will be CGATCGC, because if primer is attache to the XXXX binding site it polymerization starts and its complimentary fragment is CGATCGC.

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Statement 1: In the double-stranded DNA, only one strand serves as a template for the duplication of the new DNA.

Statement 2: Leading strand is continuously synthesised in 5’-3’ direction and lagging strand is discontinuously synthesised in 3’-5’ direction.

Statement 3: DNA polymerase reads the template strand in 5’-3’ direction.

Statement 4: Replication proceeds only in 5’-3’ direction

Choose the correct option

Match the following enzymes of DNA replication with their respective functions:
$\begin{matrix} p . D N A p o l y m e r a s e & i . H e l p s u n z i p t h e D N A d o u b l e h e l i x q . D N A l i g a s e & i i . S e a l s n i c k s b e t w e e n O k a z a k i f r a g m e n t s r . D N A h e l i c a s e & i i i . L a y s R N A p r i m e r s t o w h i c h p o l y m e r a s e c a n a d d n u c l e o t i d e s s . P r i m a s e & i v . A d d s n u c l e o t i d e s t o t h e f r e e 3^{'} e n d o f R N A p r i m e r s \end{matrix}$

Q. DNA primers are designed such that they specifically attach to the DNA fragments that we want to amplify.

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