Question

A fluorescent microscope is used

• A. For increasing resolving power.
• B. For obtaining X-ray diffraction pattern of DNA.
• C. To study cell and their components in a living state.
• D. To localise cell structure stained with fluorochrome dyes.

Answer 1

The correct option is D To localise cell structure stained with fluorochrome dyes.

A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of or in addition to, reflection and absorption to study properties of organic or inorganic substances. The "fluorescence microscope" refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescent image. So, fluorescent microscope is used to localise cell structure stained with fluorochrome dyes and not for increasing resolving power, for obtaining X-ray diffraction pattern of DNA and to study cell and their components in a living state.