wiz-icon
MyQuestionIcon
MyQuestionIcon
6
You visited us 6 times! Enjoying our articles? Unlock Full Access!
Question

. A gene was being ligated to the plasmid vector to prepare a recombinant DNA during
bacterial transformation. An exonuclease was added to the tube accidentally. How will it
affect the next step of the experiment?
What would happen if the restriction enzymes do not cut the DNA at specific recognition
sequences?

Open in App
Solution

Dear Student,

Here is the answer to you asked query-

The next step of the experiment will not be affected since the recombinant DNA molecule is circular and does not have any free ends. Therefore no substrate will be available for exonuclease enzyme to act and remove the nucleotides from the free ends of the given DNA. If the restriction enzyme does not cut the DNA at specific recogniton sequences than the desired gene can not be obtained as there will not sticky ends present and that specific character will also not be obtained.
​​​​​​

Regards

flag
Suggest Corrections
thumbs-up
0
similar_icon
Similar questions
View More
Join BYJU'S Learning Program
similar_icon
Related Videos
thumbnail
lock
Introduction to Genetics
BIOLOGY
Watch in App
Join BYJU'S Learning Program
CrossIcon