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Question
A glass plate is coated with a layer of agarose gel. A central wall and four surrounding wells (M, N, O, P) are cut on the agarose as shown in the figure on the right side. A mixture of x and y antibodies is added in the central well that react specifically with antigens X and Y, respectively. Wells M and N contain the antigen X. Wells P and O contain antigens of unknown identify. All the antigens and antibodies can diffuse through the agarose. The plate is then incubated for 48 hours. White lines of precipitates are formed in between the antigen and antibody wells as shown in the figure. Which of the following is likely to cause this?
A glass plate is coated with a layer of agarose gel. A central wall and four surrounding wells (M, N, O, P) are cut on the agarose as shown in the figure on the right side. A mixture of x and y antibodies is added in the central well that react specifically with antigens X and Y, respectively. Wells M and N contain the antigen X. Wells P and O contain antigens of unknown identify. All the antigens and antibodies can diffuse through the agarose. The plate is then incubated for 48 hours. White lines of precipitates are formed in between the antigen and antibody wells as shown in the figure. Which of the following is likely to cause this?
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Solution
The correct option is D Antigen Y in O and X in P
The antigens and antibodies can diffuse through the agarose gel and react with each other to form the precipitate. The line of precipitation formed by the antigen X in wells M and N is similar to well P. So, the antigen X is present in P. The line of precipitation by well O is different from the other wells M, N and P. Since, the line of precipitation is formed, it shows that the antigen should be Y. So, antigen Y is present in well P. Thus, the correct answer is option D.
The antigens and antibodies can diffuse through the agarose gel and react with each other to form the precipitate. The line of precipitation formed by the antigen X in wells M and N is similar to well P. So, the antigen X is present in P.
The line of precipitation by well O is different from the other wells M, N and P. Since, the line of precipitation is formed, it shows that the antigen should be Y. So, antigen Y is present in well P.
Thus, the correct answer is option D.
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