A greater scattering of light in a bacterial sample, when measured by UV spectrophotometer, indicates

Decreased bacterial growth

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Greater bacterial growth

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Stable bacterial growth

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None of the above

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Solution

The correct option is A Greater bacterial growth
The spectrophotometer measures the turbidity or optical density. Turbidity is the measure of the amount of light absorbed by a bacterial suspension. The degree of turbidity in the broth culture is directly proportional to the number of microorganism present i.e. microbial density. Thus it is a convenient and rapid method of measuring cell growth rate of an organism. Increasing turbidity of the broth medium indicates an increase in the microbial cell mass. The amount of transmitted light through turbid broth decreases with subsequent increase in the absorbance value.
So, the correct answer is 'Greater bacterial growth'

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