Question

(a) Identify steps A and B in a cycle of Polymerase Chain Reaction given below.

(b) State the specific characteristic feature of the enzyme in carrying step B. [3 marks]

Answer 1

(a) Step A represents primer annealing where the primers get attached to the specific region of the DNA. [1 mark]

Step B represents polymerization or extension of the newly synthesized DNA strand. [1 mark]

(b) In step B, the specific characteristic feature of the enzyme DNA polymerase is to add the complementary nucleotides to the growing DNA strand. [1 mark]