The correct option is A Mutation and isolation
Speciation can be defined as the process in which reproductively isolated biological populations evolve to become distinct species. A new species can be developed by mutation and isolation. There are four modes of speciation depending upon the extent to which the populations are isolated from each other viz., allopatric, peripatric, parapatric and sympatric. Hugo de Vries (proponent of mutational theory of evolution) suggested that new species arise by single mutational events.