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Question

A potted plant was taken in order to prove a factor necessary for photosynthesis. The potted plant was kept in the dark for 24 hours. One of the leaves was covered with black paper in the centre. The potted plant was then placed in sunlight for a few hours.

(c) During the starch test, why was the leaf -
(1) boiled in water
(2) boiled in methylated spirit

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Solution

During the starch test -
1. The leaf is boiled in water for a minute to kill the cells and also to remove the waxy cuticle which might interfere with the iodine test.
2. The leaf was boiled in methylated spirit over a water bath to remove the chlorophyll content of the leaf. It turns pale-white when boiled in alcohol.

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