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Question
A prerequisite in the tissue culture experiments is to sterilize the culture medium in an autoclave to prevent the microbial contamination.The desired autoclaving conditions set are
A prerequisite in the tissue culture experiments is to sterilize the culture medium in an autoclave to prevent the microbial contamination.The desired autoclaving conditions set are
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Solution
The correct option is B 1210C, 15 pounds of pressure for 15 min
Autoclaving is a technique used for sterilization of tissue culture medium to prevent contamination. The culture medium is generally sterilized by autoclaving at 121oC at 105 kPa (15 pounds) for 15-20 minutes. The duration of autoclaving is increased for larger volumes of media.
So the correct answer is '121oC, 15 pounds of pressure for 15 min'.
Autoclaving is a technique used for sterilization of tissue culture medium to prevent contamination. The culture medium is generally sterilized by autoclaving at 121oC at 105 kPa (15 pounds) for 15-20 minutes. The duration of autoclaving is increased for larger volumes of media.
So the correct answer is '121oC, 15 pounds of pressure for 15 min'.
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