A protein comprises of totally 400 amino acids given this data, asses the total number of nucleotides on the mRNA and also the number of ATP, the number of peptide bonds formed. The number of translocations and number of Ribosomes respectively are
1. On the mRNA three nucleotides form a codon for the amino acid. It has one start codon, AUG for methionine, all remaining codon for the polypeptide chain and one of three stop codon UAA, UAG, UGA which does not code for any amino acid. So, for 400 amino acid, the number of nucleotides are 400*3 = 1200 + 1 stop codon=1203 nucleotides.
2. 1 molecules of ATP are converted into AMP + 2Pi to activate 1 amino acid, so for 400 amino acid 400 ATP utilized.
3. A peptide bond is an amide type of chemical bond linking two consecutive alpha- amino acids from C1 of one alpha-amino acid and N2 of another along a peptide or protein chain.
So, for the 400 amino acid to join, 399 peptide bond is required.
4. Translocation denotes the simultaneous movement of two tRNAs together with the mRNA by one codon on the ribosome. So, for the 400 amino acid, 399 translocations are required.
5. During translation, ribosomal subunits assemble together like a sandwich on the strand of mRNA, where they proceed to attract tRNA molecules tethered to amino acids (circles). A long chain of amino acids emerges as the ribosome decodes them RNA sequence into a polypeptide or a new protein. 1 ribosome is used for the translation.
So, the correct answer is “Nucleotides=1203, ATP= 400, Peptide Bond=399, Translocations=399 and Ribosomes= 1”