The correct option is D Top layer - Milk fat
Bottom layer - Skim milk
Centrifuge is a device which is used to separate the components of a colloidal mixture based on the difference in their density.
In the centrifuge, the milk sample rotates at a very high speed, which separates the components of milk into milk fat and skim milk. Milk fat being less dense than skim milk, accumulates over the layer of skim milk. Therefore, top layer is of milk fat and bottom layer is of skim milk.