A seed cut into two halves and immersed in 0.1% triphenyl tetrazolium chloride solution. It's viability is indicated by development of _____ colour.
A seed cut into two halves and immersed in 0.1% triphenyl tetrazolium chloride solution. It's viability is indicated by development of _____ colour.
The correct option is C Pink
The TZ test is
often referred to as a quick germination test because results are usually
available within 24 to 48 hours.Seeds to be tested are
soaked in water overnight. Once imbibed, they’re cut in half with a scalpel to
expose the embryo. The halved seeds are then put in a tetrazolium chloride
solution, and observed for staining. Living tissue contains dehydrogenase
enzymes, which reduce the tetrazolium chloride to formazan, a reddish,
water-insoluble compound. This reaction only happens in or near living cells
that are releasing hydrogen as they respire, so staining indicates the seed is
living. So, the correct answer is ‘Red’.
The TZ test is often referred to as a quick germination test because results are usually available within 24 to 48 hours.Seeds to be tested are soaked in water overnight. Once imbibed, they’re cut in half with a scalpel to expose the embryo. The halved seeds are then put in a tetrazolium chloride solution, and observed for staining. Living tissue contains dehydrogenase enzymes, which reduce the tetrazolium chloride to formazan, a reddish, water-insoluble compound. This reaction only happens in or near living cells that are releasing hydrogen as they respire, so staining indicates the seed is living. So, the correct answer is ‘Red’.
