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Question

A specific recognition sequence identified by endonucleases to make cuts at specific within the DNA is:


A

Degenerate primer sequence

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B

Okazaki sequences

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C

Poly(A) tail sequences

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D

Palindromic Nucleotide sequences

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Solution

The correct option is D

Palindromic Nucleotide sequences


The correct answer is D.

The explanation for the correct answer:

  1. A palindromic sequence is a nucleic acid sequence in DNA/RNA molecules.
  2. Reading from 5' to 3' in one strand is similar to the sequence in 5' to 3' on the complementary strand.
  3. Example- BamHI, a restriction endonuclease acts on the palindromic sequence 5'-GGATCC-3'.

The explanation for the Incorrect option:

Option A:

Degenerate primers are mixtures of similar oligonucleotides that are used in a PCR.

Option B:

Okazaki fragments are short sequences of DNA nucleotides in eukaryotes that are synthesized discontinuously and later linked together by the enzyme DNA to create the lagging strand during DNA replication.

Option C:

The poly(A) tail is a long chain of adenine nucleotides that is added to a messenger RNA (mRNA) molecule during RNA processing to increase the stability of the molecule.

Final answer:

A specific recognition sequence identified by endonucleases to make cuts at specific within the DNA is Palindromic Nucleotide sequences.


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