Question

A student is given permanent slide showing budding in yeast.
The following are the steps in focussing the object under the microscope.
(i) Place the slide on the stage ; look through the eye piece and adjust the mirror and
diaphragm to get illumination
(ii) Look through eye piece and raise the objective using coarse adjustment until
object is focussed
(iii) Make the focus sharp with the help of fine adjustment
(iv) Look through the eye piece and move the slide until the object is visible
The proper sequence of steps is

• A. (i), (iii), (iv), (ii)
• B. (ii), (iii), (iv), (i)
• C. (iv), (iii), (ii), (i)
• D. (i), (iv), (ii), (iii)

Answer 1

The correct option is D (i), (iv), (ii), (iii)

The proper sequence for examining budding of yeast under microscope is- (i), (iv), (ii), (iii).

Option 'D' is correct.