A student wishes to study the cell structure under a light microscope having 10 - eyepiece and 45 - objective- He should illuminate the object by which one of the following colours of light so as to get the best possible resolution-

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Standard XIII

Biology

Introduction to Cell

A student wis...

Question

A student wishes to study the cell structure under a light microscope having $10 \times$ eyepiece and $45 \times$ objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution:

Red

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Green

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Yellow

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Blue

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Solution

The correct option is D Blue
Resolution of the microscope is inversely proportional to the wavelength of light used. Thus, the shortest wavelength of visible light will give the maximum resolution. Among yellow, green, red and blue light colour. Blue (500 nm) have the shortest wavelength so, it will give the best resolution.
Easy way to remember is to know that VIBGYOR - Violet, Indigo, Blue, Green, Yellow, Orange and Red, go in the direction of increasing frequency and thus decreasing wavelength. Thus near ultra violet light has the best resolution.

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A student wishes to study the cell structure under a light microscope having 10× eyepiece and 45× objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution:

A student wishes to study the cell structure under a light microscope having $10 \times$ eyepiece and $45 \times$ objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution: