Question

A. What is the role of Cry I Ac, Cry IIAb and Cry I Ab?
B. (i) Write the sequence of restriction site of EcoRI and give the sequence of sticky ends after EcoRI digestion

(ii) Name the source of EcoRI restriction enzyme

Answer 1

A. Cry I Ac, Cry II Ab genes are used control cotton bollworm and Cry I Ab is used to controlling corn borer.

B. (i) the sequence of restriction site of EcoRI is G'AATTC and EcoRI creates 4 nucleotide sticky ends with 5' end overhangs of AATT.

(ii) EcoRI source is bacteria E-coli of strain RY13.