Question

a) Why have DNA fragments in band 'D' moved farther away in comparison to those in band 'C'
b) Identify the anode end in the diagram.
c) What does E represent in the above diagram?

Answer 1

Given here is a diagram for the technique of gel electrophoresis in which the band of DNA is separated on the basis of the size of the strands.

• The DNA segments which are thinner move forward as they can pass through the sieves of the agar-agar Matrix.

As we can see in the diagram there are four different bands.

• The DNA is negatively charged so it moves towards the positively charged anode, therefore, the anode and is the right side of the diagram i.e B end.

In the Bands, the DNA fragments move forward because they are thinner in size as compared to the bands above them, and they can move through the matrix of the agar-agar as they are thin in size.

• E represents the standard band.