According to following diagram which of the following is not true for (A) :-
A
Km
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B
Represents binding affinity
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C
Substrate concentration
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D
Enzyme concentration
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Solution
The correct option is AKm Km is the Michaelis-Menten constant which shows the concentration of the substrate when the reaction velocity is equal to one half of the maximal velocity for the reaction. Km indicates affinity of the enzyme for its substrate. A high Km indicates low affinity while a low Km shows strong affinity. A low Km value indicates a large binding affinity, as the reaction will approach V max more rapidly. A high Km indicates that the enzyme does not bind as efficiently with the substrate, and V max will only be reached if the substrate concentration is high enough to saturate the enzyme.